Kidney diseases are a major health concern worldwide. Currently there is a large unmet need for novel biomarkers to non-invasively diagnose and monitor kidney diseases. Urinary cells are promising biomarkers and their analysis by flow cytometry has demonstrated its utility in diverse clinical settings. However, up to date this methodology depends on fresh samples, as cellular event counts and the signal-to-noise-ratio deter over time. Here we developed an easy-to-use two-step preservation method for conservation of urine samples for subsequent flow cytometry.

中文翻译：

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在尿液样本中添加甲醛释放剂咪唑烷基脲和 MOPS 缓冲液，可以延迟处理尿细胞的流式细胞分析：用于流式细胞术的尿细胞的简单两步保存方法

肾脏疾病是全世界的主要健康问题。目前，对用于非侵入性诊断和监测肾脏疾病的新型生物标志物的需求尚未得到满足。泌尿细胞是有前途的生物标志物，通过流式细胞术对其进行分析已证明其在多种临床环境中的实用性。然而，到目前为止，这种方法依赖于新鲜样本，因为细胞事件计数和信噪比会随着时间的推移而受到影响。在这里，我们开发了一种易于使用的两步保存方法，用于保存尿液样本以供后续流式细胞术使用。