Abstract

Current artificial ligaments based on polyethylene terephthalate (PET) are associated with some disadvantages due to their hydrophobicity and low biocompatibility. In this study, we aimed to modify the surface of PET using polyethylene glycol (PEG)-terminated polystyrene (PS)-linoleic nanoparticles (PLinaS-g-PEG-NPs). We accomplished that BMP-2 in two different concentrations encapsulated in nanoparticles with an efficiency of 99.71 ± 1.5 and 99.95 ± 2.8%. While the dynamic contact angle of plain PET surface reduced from 116° to 115° after a measurement periods of 10 s, that of PLinaS-g-PEG-NPs modified PET from 80° to 17.5° within 0.35 s. According to in vitro BMP2 release study, BMP-2 was released 13.12 ± 1.76% and 45.47 ± 1.78% from 0.05 and 0.1BMP2-PLinaS-g-PEG-NPs modified PET at the end of 20 days, respectively. Findings from this study revealed that BMP2-PLinaS-g-PEG-NPs has a great potential to improve the artificial PET ligaments, and could be effectively applied for ACL reconstruction.

摘要

目前基于聚对苯二甲酸乙二醇酯 (PET) 的人工韧带由于其疏水性和低生物相容性而存在一些缺点。在这项研究中，我们旨在使用聚乙二醇 (PEG) 封端的聚苯乙烯 (PS)-亚油酸纳米粒子 (PLinaS-g-PEG-NPs) 修饰 PET 的表面。我们实现了两种不同浓度的 BMP-2 封装在纳米颗粒中，效率分别为 99.71 ± 1.5 和 99.95 ± 2.8%。在 10 秒的测量周期后，普通 PET 表面的动态接触角从 116° 降低到 115°，而 PLinaS-g-PEG-NPs 改性 PET 的动态接触角在 0.35 秒内从 80° 降低到 17.5°。根据体外BMP2 释放研究，BMP-2 在 20 天结束时分别从 0.05 和 0.1BMP2-PLinaS-g-PEG-NPs 修饰的 PET 中释放 13.12 ± 1.76% 和 45.47 ± 1.78%。本研究结果表明，BMP2-PLinaS-g-PEG-NPs 具有改善人工 PET 韧带的巨大潜力，可有效应用于 ACL 重建。