The aim of this study was to determine the actual state of the photosynthetic apparatus and exhibit distinguishable differences in the chlorophyll fluorescence (ChlF) components in different seedling ages of M. oiwakensis plants subjected to different light intensity (LI). Potted 6-month-old greenhouse seedlings and field collected 2.4-year-old seedlings with 5 cm heights were selected and randomly separated into seven groups for photosynthesis measurements illuminated with 50, 100 (assigned as low LI), 300, 500, 1,000 (as moderate LI), 1,500 and 2,000 (as high LI) μmol m–2 s–1 photosynthetic photon flux density (PPFD) treatments. n 6-month-old seedlings, as LI increased from 50 to 2,000 PPFD, the values of non-photochemical quenching and photo-inhibitory quenching (qI) increased but potential quantum efficiency of PSII (Fv/Fm) and photochemical efficiency of photosystem II (ΦPSII) values decreased. High electron transport rate and percentage of actual PSII efficiency by Fv/Fm values were observed in 2.4-year-old seedlings at high LI conditions. Furthermore, higher ΦPSII was detected under low LI conditions, with lower energy-dependent quenching (qE) and qI values and photo-inhibition % decreased as well. However, qE and qI increased as ΦPSII decreased and photo-inhibition% increased under high LI treatments. These results could be useful for predicting the changes in growth and distribution of Mahonia species grown in controlled environments and open fields with various combinations of varying light illuminations, and ecological monitoring of their restoration and habitat creation is important for provenance conservation and helps to formulate better conservation strategies for the seedlings.

中文翻译：

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不同苗龄马鞭草光诱导过程中光合作用和叶绿素荧光的响应

本研究的目的是确定光合装置的实际状态，并在受不同光强度 (LI) 影响的 M. oiwakensis 植物的不同苗龄中表现出叶绿素荧光 (ChlF) 成分的明显差异。选择盆栽 6 个月大的温室幼苗和田间采集的 2.4 年生苗高 5 cm 的幼苗，随机分为七组进行光合作用测量，光照为 50、100（指定为低 LI）、300、500、1,000（作为中度 LI)，1,500 和 2,000（作为高 LI）μmol m–2 s–1 光合光子通量密度 (PPFD) 处理。n 6 个月大的幼苗，随着 LI 从 50 增加到 2,000 PPFD，非光化学猝灭和光抑制猝灭（qI）值增加，但PSII潜在量子效率（Fv/Fm）和光系统II光化学效率（ΦPSII）值降低。在高 LI 条件下，在 2.4 年生的幼苗中观察到高电子传输速率和 Fv/Fm 值的实际 PSII 效率百分比。此外，在低 LI 条件下检测到更高的 ΦPSII，能量依赖性猝灭 (qE) 和 qI 值更低，光抑制百分比也降低。然而，在高 LI 处理下，qE 和 qI 随着 ΦPSII 的降低和光抑制百分比的增加而增加。这些结果可用于预测在受控环境和开阔田野中生长的 Mahonia 物种的生长和分布变化，以及不同光照度的各种组合，