L-DOPA is an amino acid that is used as a treatment for Parkinson’s disease. A simple enzymatic synthesis method of L-DOPA had been developed using bacterial L-tyrosine phenol-lyase (Tpl). This review describes research on screening of bacterial strains, culture conditions, properties of the enzyme, reaction mechanism of the enzyme, and the reaction conditions for the production of L-DOPA. Furthermore, molecular bleeding of constitutively Tpl-overproducing strains is described, which were developed based on mutations in a DNA binding protein, TyrR, which controls the induction of tpl gene expression.

中文翻译：

---

使用细菌酶酪氨酸苯酚裂解酶生产左旋多巴的研究概况

L-DOPA 是一种氨基酸，可用于治疗帕金森病。使用细菌 L-酪氨酸苯酚裂解酶 (Tpl) 开发了一种简单的 L-DOPA 酶促合成方法。本文综述了细菌菌株的筛选、培养条件、酶的性质、酶的反应机理以及生产左旋多巴的反应条件等方面的研究。此外，还描述了组成型 Tpl 过度生产菌株的分子出血，这些菌株是基于 DNA 结合蛋白 TyrR 的突变而开发的，TyrR 控制着tpl基因表达的诱导。