BACKGROUND Multidrug resistance is the biggest barrier on the way to chemotherapy for lung adenocarcinoma (LUAD). For some LUAD patients with cisplatin (DDP) resistance and poor prognoses, the authors put forward RNA nanoparticles (NPs) carrying miR-301b-3p Inhibitor. METHODS The NPs were composed of miR-301b-3p, A549 aptamer (A549apt), and Cyanine 5 in a bottom-up manner with a 3-way-junction (3WJ) structure. Diameter, assembly process, and morphology of NPs were observed by Dynamic Light Scattering, Native-Polyacrylamide Gel Electrophoresis, and Atomic Force Microscopy. Cell internalisation, toxicity, proliferation, migration, invasion, and apoptosis were assayed by confocal laser scanning microscope, CCK8, colony formation assay, Transwell, western blot, and flow cytometry. RESULTS 3WJ-apt-miR was evenly distributed, with diameter of 19.61 ± 0.49 nm and triangular branching structures. The accurate delivery of this NP in vivo was ensured by A549 aptamer featuring specific targeting, with smaller side effects than traditional chemotherapy. Such nanomaterials were effectively internalized by cancer cells, with normal cell activity intact. Cancer cell proliferation, invasion, and migration were suppressed, and DDP sensitivity was enhanced, causing DNA damage and facilitating apoptosis of DDP-resistant cells. CONCLUSION Based on RNA self-assembling, the authors researched the effect of miRNA on DDP sensitivity in LUAD regarding gene regulation. 3WJ-apt-miR paves the way for clinical tumour therapy.

中文翻译：

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携带miR-301b-3p抑制剂的RNA纳米粒子的制备、表征及体外抑癌作用。

背景技术多药耐药是肺腺癌（LUAD）化疗的最大障碍。针对一些顺铂（DDP）耐药且预后不佳的LUAD患者，作者提出了携带miR-301b-3p Inhibitor的RNA纳米粒（NPs）。方法 NPs 由 miR-301b-3p、A549 适体 (A549apt) 和 Cyanine 5 以自下而上的方式组成，具有三向连接 (3WJ) 结构。通过动态光散射、天然聚丙烯酰胺凝胶电泳和原子力显微镜观察 NP 的直径、组装过程和形态。通过共聚焦激光扫描显微镜、CCK8、集落形成测定、Transwell、蛋白质印迹和流式细胞术测定细胞内化、毒性、增殖、迁移、侵袭和凋亡。结果 3WJ-apt-miR分布均匀，直径为19。61 ± 0.49 nm 和三角形分支结构。A549 适配体具有特定靶向性，与传统化疗相比副作用更小，确保了该 NP 在体内的准确递送。这种纳米材料被癌细胞有效地内化，正常细胞活性完好无损。癌细胞增殖、侵袭和迁移受到抑制，DDP敏感性增强，造成DNA损伤，促进DDP耐药细胞凋亡。结论 基于RNA自组装，作者研究了miRNA对LUAD基因调控中DDP敏感性的影响。3WJ-apt-miR为临床肿瘤治疗铺平了道路。副作用比传统化疗小。这种纳米材料被癌细胞有效地内化，正常细胞活性完好无损。癌细胞增殖、侵袭和迁移受到抑制，DDP敏感性增强，造成DNA损伤，促进DDP耐药细胞凋亡。结论 基于RNA自组装，作者研究了miRNA对LUAD基因调控中DDP敏感性的影响。3WJ-apt-miR为临床肿瘤治疗铺平了道路。副作用比传统化疗小。这种纳米材料被癌细胞有效地内化，正常细胞活性完好无损。癌细胞增殖、侵袭和迁移受到抑制，DDP敏感性增强，造成DNA损伤，促进DDP耐药细胞凋亡。结论 基于RNA自组装，作者研究了miRNA对LUAD基因调控中DDP敏感性的影响。3WJ-apt-miR为临床肿瘤治疗铺平了道路。作者研究了 miRNA 对 LUAD 基因调控中 DDP 敏感性的影响。3WJ-apt-miR为临床肿瘤治疗铺平了道路。作者研究了 miRNA 对 LUAD 基因调控中 DDP 敏感性的影响。3WJ-apt-miR为临床肿瘤治疗铺平了道路。