Five strains of Drouetiella (ACKU666, 667, 668, 669 and 670) were isolated from gravels in water, stone monument and coastal mudflat in Korea, and were studied using morphological and molecular traits. All five strains had thin and simple trichomes and exhibited false branching. From these strains, four strains (ACKU666, 667, 668 and 669) exhibited similar cell lengths with reddish–brown colored cells such as Drouetiella lurida. The 16S rRNA gene phylogeny showed the four strains formed a clade with Drouetiella lurida, and their DNA similarity was calculated to be 99.1–100%. The color of strain ACKU670 appeared to be in bright blue–green color like Drouetiella fasciculata, and their thylakoids showed a parietal arrangement, which is a characteristic feature of the family Oculatellaceae. Strain ACKU670 turned out to be a sister clade to the D. lurida according to the phylogenetic analysis of the 16S rRNA gene. The 16–23S rRNA internal transcribed spacer secondary folding structure (D1–D1′, Box-B and V3 helices) confirmed the uniqueness of strain ACKU670, therefore indicating differences from the related species. Considering all the results, we described our strain ACKU670 as Drouetiella epilithica sp. nov. in accordance with the International Code of Nomenclature for Algae, Fungi and Plants.

中文翻译：

---

Drouetiella epilithica sp. 十一月 和 Drouetiella lurida（Oculatellaceae，Synechococcales）基于多相方法在韩国分离出来

从韩国水体、石碑和沿海泥滩的砾石中分离出 5 个Drouetiella菌株（ACKU666、667、668、669 和 670），并对其形态和分子特征进行了研究。所有五个菌株都具有薄而简单的毛状体，并表现出假分枝。在这些菌株中，四种菌株（ACKU666、667、668 和 669）表现出相似的细胞长度，其中细胞呈红棕色，例如Drouetiella lurida。16S rRNA基因系统发育显示4个菌株与Drouetiella lurida形成一个进化枝，其DNA相似性为99.1%~100%。菌株 ACKU670 的颜色看起来像Drouetiella fasciculata一样呈明亮的蓝绿色，它们的类囊体呈现顶叶排列，这是眼眼科的特征。菌株 ACKU670 被证明是D 的姐妹进化枝。根据16S rRNA基因的系统发育分析。16-23S rRNA 内部转录间隔区二级折叠结构（D1-D1'、Box-B 和 V3 螺旋）证实了菌株 ACKU670 的独特性，因此表明与相关物种的差异。考虑到所有结果，我们将我们的菌株 ACKU670 描述为Drouetiella epilithica sp。十一月 根据《国际藻类、真菌和植物命名法》。