Enhancing the stability and sensitivity of electrochemical biosensors is highly significant for their practical application. Herein, inspired by the formation of mussel foot protein, we proposed a strategy to construct a hemoglobin-based biosensor for hydrogen peroxide detection using a hydrophobic ionic liquid (HIL) coordination assisted microfluidic technology. The active layer HIL@Hb was achieved by mixing BBimPF₆ (HIL) and Hb via a microfluidic channel, in which HIL helps to maintain the conformational dynamic mobility of hemoglobin (Hb), while the coordination process in a microfluidic reactor prevents aggregation of Hb. Further, the electrode surface was modified with ultra-thin MXene-Ti₃C₂ nanosheets to ensure the effective adhesion of active layer and collection of sensing signals, thus improving the sensitivity of the sensor by synergistic catalysis. Experimental results demonstrate that our designed sensor has good repeatability and stability, which can retain 93% of the initial current response after 30 uses and about 90.11% of its primary current response to H₂O₂ after 30 days. And it has a good linear response range of 1.996–27.232 μM, detection limit reaching 1.996 nM (signal-to-noise ratio, S/N = 3), sensitivity of 52.08 μA·μM⁻¹·cm⁻². Overall, this research offers a facile and effective strategy for constructing a stable biosensor to effectively detect hydrogen peroxide.

提高电化学生物传感器的稳定性和灵敏度对其实际应用具有重要意义。在此，受贻贝足蛋白形成的启发，我们提出了一种策略，使用疏水性离子液体 (HIL) 配位辅助微流控技术构建用于过氧化氢检测的基于血红蛋白的生物传感器。活性层 HIL@Hb 是通过微流体通道混合 BBimPF 6 (HIL) 和 Hb 实现的，其中 HIL 有助于维持血红蛋白 (Hb) 的构象动态流动性，而微流体反应器中的配位过程可防止 Hb_聚集. 此外，电极表面用超薄 MXene-Ti ₃ C _{2进行了修饰}纳米片确保活性层的有效附着和传感信号的收集，从而通过协同催化提高传感器的灵敏度。实验结果表明，我们设计的传感器具有良好的重复性和稳定性，在使用30次后仍能保持93%的初始电流响应，在30天后仍能保持其对H 2 O 2 的初级电流响应的约_90.11 % _。具有良好的线性响应范围1.996~27.232 μM，检出限达到1.996 nM（信噪比，S/ N  = 3），灵敏度52.08 μA·μM ^-1 ·cm ^-2。总的来说，这项研究为构建稳定的生物传感器以有效检测过氧化氢提供了一种简便有效的策略。