Seasonal variation in algae biomass productivity is a significant obstacle to the economical production of fuels and chemicals from algae biomass. Long-term storage is necessary to guarantee a constant supply to a conversion facility and harvested microalgae biomass (20% solids) is subject to degradation immediately upon harvesting as a result of aerobic instability. Ensiling has been successfully used to preserve microalgal biomass, but biomass attributes that contribute to successful storage are poorly understood. Storage studies of Monoraphidium sp. biomass indicate a strong correlation between nitrogen management in algae cultivation and stability of post-harvest algae biomass in 30 days of anaerobic storage. Algae cultivated with periodic nitrogen addition stored poorly (> 20% loss, dry basis), while algae biomass from cultures that were allowed to continue growing after nitrogen depletion was more stable in storage (8% loss, dry basis). A follow-up study aimed at exploring the relationship between nitrogen management in cultivation and stability of post-harvest algae biomass stored Monoraphidium biomass cultivated in nitrogen deplete and nitrogen replete conditions, as well as with blends of each in varying ratios. Monoraphidium biomass from nitrogen replete cultivations experienced the largest degradation (24%, dry basis), while nitrogen deplete biomass experienced the least (10%, dry basis). Dry matter loss experienced during storage of blends was positively correlated with the amount of nitrogen replete biomass that a blend contained. The composition of the post-storage algae microbial community was also strongly affected by cultivation conditions, with Clostridia species being more prevalent in stored biomass obtained from nitrogen replete cultivations. Nitrogen management has long been known to influence algae biomass productivity and biochemical composition; here, we demonstrate that it also strongly influences the stability of post-harvest algae biomass in anaerobic storage.

中文翻译：

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栽培中氮肥管理对收获后单针藻稳定性和微生物群落的影响。藻类生物量

藻类生物质生产力的季节性变化是从藻类生物质经济地生产燃料和化学品的重大障碍。长期储存对于保证转化设施的持续供应是必要的，并且收获的微藻生物质（20%固体）在收获后由于需氧不稳定而立即降解。青贮已成功用于保存微藻生物量，但有助于成功储存的生物量属性却知之甚少。Monoraphidium sp. 的储存研究。生物量表明藻类培养中的氮管理与收获后藻类生物量在 30 天厌氧储存中的稳定性之间存在很强的相关性。通过定期添加氮培养的藻类储存不良(损失大于20％，干基)，而来自在氮消耗后允许继续生长的培养物的藻类生物量在储存中更稳定(损失8％，干基)。后续研究旨在探索培养中的氮管理与收获后藻类生物质储存的稳定性之间的关系，这些藻类生物质在贫氮和富氮条件下以及不同比例的混合物中培养。来自全氮栽培的单针藻生物量经历了最大的退化（24％，干基），而氮消耗生物量经历了最小（10％，干基）。混合物储存期间经历的干物质损失与混合物所含的富含氮的生物质的量呈正相关。储存后藻类微生物群落的组成也受到培养条件的强烈影响，梭菌属物种在从充满氮的培养中获得的储存生物量中更为普遍。人们早就知道氮管理会影响藻类生物量生产力和生化成分；在这里，我们证明它也强烈影响收获后藻类生物量在厌氧储存中的稳定性。