Abstract

The goal of the study was molecular typing of current Neisseria gonorrhoeae clinical isolates in Russia for the identification of epidemiologically significant NG-MAST and MLST sequence types carrying a “mosaic” penA gene allele with reduced sensitivity to third-generation cephalosporins, which are recommended as first-line antimicrobials for gonococcal infection therapy. The study included 326 N. gonorrhoeae clinical isolates collected in Russia in 2018–2020. NG-MAST typing was based on sequencing of variable regions of porB and tbpB genes. MLST typing was performed on the basis of the nucleotide sequences of seven housekeeping genes: abcZ, adk, aroE, fumC, gdh, pdhC, and pgm. The penA gene structure was analyzed according to the Sanger protocol. Molecular typing assigned the examined N. gonorrhoeae clinical isolates to 110 NG-MAST and 41 MLST different sequence types. The penA gene sequencing revealed a “mosaic”-type XXXIV allele encoding penicillin-binding protein 2 with reduced affinity to cephalosporins in 7 (2.1%) clinical isolates. Comparison of these results showed the presence of a “mosaic” penA allele in clinical isolates related to ST_NG-MAST2212/ST_MLST1901 (n = 1); ST_NG-MAST3149/ST_MLST1901 (n = 1); ST_NG-MAST5622/ ST_MLST14011 (n = 1), and ST_NG-MAST10025/ST_MLST1902 (n = 4) sequence types. Phylogenetic analysis has linked all named sequence types to the internationally spread, multiresistance clone ST_NG-MAST1407/ ST_MLST1901, which is a public health concern globally. The study showed the diversity of current N. gonorrhoeae sequence types carrying the “mosaic” penA gene allele, probably as a result of novel mutational events in the porB, adk, and fumC genes taken into account by the NG-MAST and MLST protocols.

中文翻译：

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携带 penA 基因“镶嵌”等位基因的俄罗斯淋病奈瑟菌临床分离株中 NG-MAST 和 MLST 序列类型的多样性

摘要

该研究的目的是对俄罗斯目前的淋病奈瑟菌临床分离株进行分子分型，以鉴定具有流行病学意义的 NG-MAST 和 MLST 序列类型，这些序列类型携带对第三代头孢菌素的敏感性降低的“镶嵌” penA基因等位基因，被推荐为用于淋球菌感染治疗的一线抗菌药物。该研究包括2018-2020 年在俄罗斯收集的326株淋病奈瑟菌临床分离株。NG-MAST 分型基于porB和tbpB基因可变区的测序。MLST 分型是根据七个管家基因的核苷酸序列进行的：abcZ、adk、aroE、fumC、gdh、pdhC和pgm。根据Sanger方案分析penA基因结构。分子分型将检查的淋病奈瑟球菌临床分离株分配给 110 种 NG-MAST 和 41 种 MLST 不同序列类型。penA基因测序揭示了一个“马赛克”型 XXXIV 等位基因编码青霉素结合蛋白 2，在 7 个 (2.1%) 临床分离株中对头孢菌素的亲和力降低。_{这些结果的比较表明，在与 ST NG-MAST} 2212/ST _MLST 1901（n = 1）相关的临床分离株中存在“马赛克” penA等位基因；英石_NG-MAST 3149/ST _MLST 1901（n = 1）；ST _NG-MAST 5622/ST _MLST 14011（n = 1）和 ST _NG-MAST 10025/ST _MLST 1902（n = 4）序列类型。系统发育分析已将所有命名序列类型与国际传播的多抗性克隆 ST _NG-MAST 1407 / ST _MLST 1901 联系起来，这是全球公共卫生问题。该研究显示了当前携带“镶嵌” penA基因等位基因的淋病奈瑟球菌序列类型的多样性，这可能是porB、adk中新突变事件的结果, 以及NG-MAST 和 MLST 协议考虑的fumC基因。