Abstract

Objective: To compare the repertoire of proteins of the human hemostatic system and fragments mimicking these proteins in the proteins of influenza A/H1N1 viruses and coronaviruses. Material and methods. Influenza viruses A/H1N1 (A/Brevig Mission/1/18), A/St. Petersburg /RII04/2016 (H1N1)pdm09, coronaviruses SARS-CoV and SARS-CoV-2 (strain Wuhan-Hu-1) were used for comparative computer analysis. The sources of the primary structures of proteins of the analyzed viruses and 41 proteins of the human hemostatic system were publicly available Internet databases, respectively, www.ncbi.nlm.nih.gov and www.nextprot.org. The search for homologous sequences in the structure of viral proteins and hemostatic proteins was carried out by comparing fragments of 12 amino acids in length, taking as related those that showed identity at ≥8 positions. Results. Comparative analysis of the repertoire of cellular proteins of the hemostatic system and fragments mimicking these proteins in the structure of proteins of viruses A/H1N1 1918, A(H1N1)pdm09 isolated in 2016, SARS-CoV and SARS-CoV-2, showed a significant difference between SARS-CoV-2 and analyzed viruses. In the protein structure of the SARS-CoV-2 virus, mimicry was revealed for almost all analyzed hemostasis proteins. As for the comparison of viruses A/H1N1 1918, A(H1N1)pdm09 2016 and SARS-CoV, the influenza virus A/H1N1 1918 and SARS-CoV are the closest in the repertoire of hemostatic proteins. Conclusion. Obtained bioinformatic analysis data can serve as a basis for further study of the role of homologous fragments in the regulation of hemostasis of the host organism.

中文翻译：

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模拟 A/H1N1 病毒和冠状病毒蛋白质片段的人类止血蛋白片段比较

摘要

目的：比较人类止血系统的蛋白质库以及在甲型流感/H1N1 病毒和冠状病毒的蛋白质中模拟这些蛋白质的片段。材料与方法. 流感病毒 A/H1N1 (A/Brevig Mission/1/18), A/St. Petersburg /RII04/2016 (H1N1)pdm09、冠状病毒 SARS-CoV 和 SARS-CoV-2（毒株 Wuhan-Hu-1）用于比较计算机分析。所分析病毒的蛋白质一级结构和人体止血系统的 41 种蛋白质的来源分别是 www.ncbi.nlm.nih.gov 和 www.nextprot.org 的公开互联网数据库。病毒蛋白和止血蛋白结构中的同源序列搜索是通过比较长度为 12 个氨基酸的片段进行的，将那些在 ≥ 8 个位置显示同一性的片段视为相关。结果. 对止血系统细胞蛋白和模拟这些蛋白的片段在 A/H1N1 1918 病毒、2016 年分离的 A(H1N1)pdm09、SARS-CoV 和 SARS-CoV-2 的蛋白结构中的比较分析表明SARS-CoV-2 和分析的病毒之间存在显着差异。在 SARS-CoV-2 病毒的蛋白质结构中，几乎所有分析的止血蛋白都显示出拟态。至于病毒A/H1N1 1918、A(H1N1)pdm09 2016和SARS-CoV的比较，流感病毒A/H1N1 1918和SARS-CoV在止血蛋白库中最接近。结论。获得的生物信息学分析数据可以作为进一步研究同源片段在宿主生物体止血调节中的作用的基础。