Polyethersulfone (PES) membranes are widely used in medical devices, especially intravascular devices such as intravascular bioartificial pancreases. In the current work, the pure PES and PES-pyrolytic carbon (PyC) composite membranes were synthesized and permeability studies were conducted. In addition, the cytocompatibility and hemocompatibility of the pure PES and PES-PyC membranes were investigated. These materials were characterized using peripheral blood mononuclear cell (PBMC) activation, platelet activation, platelet adhesion, ß-cell viability and proliferation, and ß-cell response to hyperglycemia. The results showed that platelet activation decreased from 87.3% to 27.8%. Any alteration in the morphology of sticking platelets was prevented, and the number of attached platelets decreased by modification with PyC. The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay corroborated that PBMC activation was encouraged by the PyC-modified PES membrane surface. It can be concluded that PES-modified membranes show higher hemocompatibility than pure PES membranes. ß-cells cultured on all the three membranes displayed a lower rate of proliferation although the cells on the PES-PyC (0.1 wt. %) membrane indicated a slightly higher viability and proliferation than those on the pure PES and PES-PyC (0.05 wt. %) membranes. It shows that the PES-PyC (0.1 wt. %) membrane possesses superior cytocompatibility over the other membranes.

中文翻译：

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聚醚砜-热解碳复合膜在人工胰腺中的生物相容性评价。

聚醚砜（PES）膜广泛应用于医疗器械，尤其是血管内器械，如血管内生物人工胰腺。在目前的工作中，合成了纯 PES 和 PES-热解碳 (PyC) 复合膜，并进行了渗透性研究。此外，还研究了纯 PES 和 PES-PyC 膜的细胞相容性和血液相容性。这些材料使用外周血单核细胞 (PBMC) 激活、血小板激活、血小板粘附、ß 细胞活力和增殖以及 ß 细胞对高血糖的反应进行表征。结果显示，血小板活化从 87.3% 下降到 27.8%。阻止了粘附血小板形态的任何改变，并且通过 PyC 修饰减少了粘附血小板的数量。3-(4, 5-二甲基噻唑-2-基)-2, 5-二苯基四唑溴化物测定证实了 PyC 修饰的 PES 膜表面促进了 PBMC 活化。可以得出结论，PES 修饰膜显示出比纯 PES 膜更高的血液相容性。尽管 PES-PyC（0.1 wt.%）膜上的细胞比纯 PES 和 PES-PyC（0.05 wt. . %) 膜。它表明 PES-PyC (0.1 wt. %) 膜具有优于其他膜的细胞相容性。可以得出结论，PES 修饰膜显示出比纯 PES 膜更高的血液相容性。尽管 PES-PyC（0.1 wt.%）膜上的细胞比纯 PES 和 PES-PyC（0.05 wt. . %) 膜。它表明 PES-PyC (0.1 wt. %) 膜具有优于其他膜的细胞相容性。可以得出结论，PES 修饰膜显示出比纯 PES 膜更高的血液相容性。尽管 PES-PyC（0.1 wt.%）膜上的细胞比纯 PES 和 PES-PyC（0.05 wt. . %) 膜。它表明 PES-PyC (0.1 wt. %) 膜具有优于其他膜的细胞相容性。