ABSTRACT

Lophodermium species are well known to be among the dominant endophytic fungi colonizing pine needles. The occurrence of two species with different life strategies on different Pinus trees across Slovakia was detected by PCR using species-specific primers. In general, the commensalistic species L. pinastri was more frequent than parasitic L. seditiosum. There were no significant differences observed between urban and natural environments, but natural environments have more frequent colonization by L. pinastri and less frequent were trees negatively tested to Lophodermium colonization. Among the most frequently sampled trees were non-native P. nigra and native P. sylvestris. A significant difference in Lophodermium incidence was detected between these two species in a natural environment, with increased frequency of L. pinastri on native Scots pine. In addition to one clade of L. pinastri, culture based species identification confirmed the presence of L. corconticum and an undescribed clade of L. seditiosum that is not sensitive to used PCR primers for the species detection.

摘要

众所周知，Lophodermium物种是在松针上定殖的主要内生真菌之一。使用物种特异性引物通过 PCR 检测到斯洛伐克不同松树上两种具有不同生活策略的物种的出现。一般来说，共生物种L. pinastri比寄生L. seditiosum更常见。城市环境和自然环境之间没有观察到显着差异，但自然环境中L. pinastri的定殖频率更高，而树木对Lophodermium定植的阴性测试频率更低。在最常采样的树木中，有非本地的P. nigra和本地的樟子松。在自然环境中检测到这两个物种的Lophodermium发生率存在显着差异，本地苏格兰松上L. pinastri的发生频率增加。除了L. pinastri的一个进化枝外，基于培养的物种鉴定证实了L. corconticum和一个未描述的L. seditiosum进化枝的存在，该进化枝对用于物种检测的 PCR 引物不敏感。