The development of peroxidase mimics with enhanced peroxidase-like activity is critical to building a convenient and fast glucose colorimetric sensor. Herein, a porphyrin-based conjugated microporous polymer (FePCMP) was synthesized through a Pd-/CuI catalyzed Sonogashira coupling reaction. The FePCMP exhibited specific and superior POD-like activity evaluated by the fast oxidation of 3,3$\prime$,5,5$\prime$-tetramethylbenzidine (a chromogenic substrate, TMB) to form the blue product (oxTMB) in the presence of H₂O₂. The outstanding POD-like activity is mainly ascribed to the Fe-N₄ active sites and the cross-linked porous framework of FePCMP. Furthermore, the FePCMP was applied in selective colorimetric detection of glucose through a glucose oxidase biocatalytic cascade reaction with a low detection limit (LOD) of 0.031 $\mu$M in the linear range of 0.2–5 $\mu$M. This study not only provided a new method for the design and synesis of specific POD-like nanozymes, but also the prepared FePCMP can be used as a POD-like enzyme for the colorimetric detection of other molecules, such as cholesterol, acetylcholine, etc.

开发具有增强的过氧化物酶样活性的过氧化物酶模拟物对于构建方便快速的葡萄糖比色传感器至关重要。在此，通过 Pd-/CuI 催化的 Sonogashira 偶联反应合成了基于卟啉的共轭微孔聚合物（FePCMP）。通过 3,3 的快速氧化评估，FePCMP 表现出特异且优异的 POD 样活性$\prime$,5,5$\prime$_{-四甲基联苯胺（显色底物，TMB）在 H 2} O ₂存在下形成蓝色产物（oxTMB）。优异的类POD活性主要归因于FePCMP的Fe-N ₄活性位点和交联多孔骨架。此外，FePCMP 通过葡萄糖氧化酶生物催化级联反应应用于葡萄糖的选择性比色检测，检测限 (LOD) 为 0.031$\mu$M在0.2-5的线性范围内$\mu$该研究不仅为特异性POD类纳米酶的设计和合成提供了新的方法，而且制备的FePCMP可作为POD类酶用于其他分子的比色检测，如胆固醇、乙酰胆碱、 ETC。