Abstract

Aim

To modify the techno-functional properties of mung bean protein isolate (MBPI) by high-intensity pulsed electric field (HIPEF) treatment and to apply the treated MBPI for encapsulation of Asian seabass oil (ASO).

Methods

MBPI was prepared using isoelectric precipitation. HIPEF was applied to MBPI solutions at 25 kV/cm with varying pulse numbers (0–400). Physicochemical properties and structure of MBPI were assessed. ASO microcapsules prepared using HIPEF-treated protein as wall material was characterised and tested for storage stability.

Results

Solubility, surface hydrophobicity, total sulfhydryl content, and emulsifying property of MBPI increased and β-sheets and α-helix were altered after HIPEF treatment at pulse number of 300. ASO microcapsules possessing spherical shape with surface indentations had EE of 72.07 ± 5.08%. ASO capsules had lower lipid oxidation than the control during storage.

Conclusion

HIPEF improved techno-functional properties of treated MBPI. Treated MBPI could be used as wall material for encapsulation of fish oils.

中文翻译：

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高强度脉冲电场处理的绿豆分离蛋白：特性及其在鲈鱼油包封中的应用

摘要

目的

通过高强度脉冲电场 (HIPEF) 处理来改变绿豆分离蛋白 (MBPI) 的技术功能特性，并将处理后的 MBPI 用于亚洲鲈鱼油 (ASO) 的包封。

方法

MBPI 采用等电沉淀法制备。HIPEF 在 25 kV/cm 下以不同的脉冲数 (0–400) 应用于 MBPI 溶液。评估了 MBPI 的物理化学性质和结构。使用 HIPEF 处理的蛋白质作为壁材料制备的 ASO 微胶囊进行了表征并测试了储存稳定性。

结果

在脉冲数为300的HIPEF处理后，MBPI的溶解度、表面疏水性、总巯基含量和乳化性能增加，β-折叠和α-螺旋发生改变。具有表面凹痕的球形形状的ASO微胶囊的EE为72.07±5.08%。ASO 胶囊在储存期间的脂质氧化低于对照。

结论

HIPEF 改善了经过处理的 MBPI 的技术功能特性。处理后的MBPI可用作包封鱼油的壁材料。