Glutathione transferase enzymes (GSTs) are believed to be a major detoxification system in helminth parasites and have been associated with immunomodulation of the host response. Echinococcus granulosus sensu lato (s.l.) is a cestode parasite known to express at least five different GSTs, but no Omega-class enzymes have been reported in this parasite or in any other cestode. Herein we report the identification of a new member of the GST superfamily in E. granulosus s.l., which is phylogenetically related to the Omega-class: EgrGSTO. Through mass spectrometry, we showed that the 237 amino acids protein EgrGSTO is expressed by the parasite. Moreover, we identified homologues of EgrGSTO in other eight members of the Taeniidae family, including E. canadensis, E. multilocularis, E. oligarthrus, Hydatigera taeniaeformis, Taenia asiatica, T. multiceps, T. saginata and T. solium. A manual sequence inspection and rational modification yielded eight Taeniidae’s GSTO sequences, each one encoding for a 237 aa polypeptide showing 80.2% overall identity. To the best of our knowledge, this is the first description of genes encoding for Omega-class GSTs in worms belonging to the Taeniidae family -that at least in E. granulosus s.l. is expressed as a protein- suggesting the gene encodes for a functional protein.

谷胱甘肽转移酶 (GST) 被认为是蠕虫寄生虫中的主要解毒系统，并且与宿主反应的免疫调节有关。Echinococcus granulosus sensu lato (sl) 是一种已知表达至少五种不同 GST 的绦虫寄生虫，但在这种寄生虫或任何其他绦虫中均未报道 Omega 类酶。在此我们报告了E. granulosus sl中 GST 超家族新成员的鉴定，它在系统发育上与 Omega 类相关：EgrGSTO。通过质谱法，我们发现寄生虫表达了 237 个氨基酸的蛋白质 EgrGSTO。此外，我们在 Taeniidae 家族的其他八个成员中发现了 EgrGSTO 的同系物，包括E. canadensis，E. multilocularis、E. oligarthrus、Hydatigera taeniaeformis、Taenia asiatica、T. multiceps、T. saginata和T. solium。手动序列检查和合理修改产生八个 Taeniidae 的 GSTO 序列，每个序列编码 237 个氨基酸的多肽，显示 80.2% 的总体同一性。据我们所知，这是首次描述在属于绦虫科的蠕虫中编码 Omega 级 GST 的基因——至少在E. granulosus sl 中表达为蛋白质——表明该基因编码功能性蛋白质.