We examined the growth tolerance, bioremoval efficacy, and metabolomic profiles of the cellular responses of Chlorella pyrenoidosa to phenol and 4-fluorophenol. We found that C. pyrenoidosa can tolerate exposure to 100 mg/L of phenol and 4-fluorophenol, and the growth of algal cells had a significant hormesis of inhibition first and then promotion. Up to 70% bioremoval of phenol and 4-fluorophenol may occur after 240 h of treatment. Superoxide dismutase (SOD) and catalase (CAT) activities, malondialdehyde (MDA) content, and reactive oxygen species (ROS) in algal cells in the phenol- and 4-fluorophenol-treated groups were similar or lower than in the control group. Furthermore, photosynthetic pigment and glycerophospholipid contents were significantly upregulated in both phenol- and 4-fluorophenol-treated groups, as indicated by the metabolomic analysis of C. pyrenoidosa, resulting in the vigorous growth of algal cells compared to the control group. Therefore, C. pyrenoidosa can be an excellent biosorbent for phenol and 4-fluorophenol.

我们检查了蛋白核小球藻对苯酚和 4-氟苯酚的细胞反应的生长耐受性、生物去除功效和代谢组学特征。我们发现C. pyrenoidosa能耐受100 mg/L的苯酚和4-氟苯酚暴露，藻细胞生长有显着的先抑制后促进的毒效效应。处理 240 小时后，苯酚和 4-氟苯酚的生物去除率可能高达 70%。苯酚和 4-氟苯酚处理组藻类细胞中的超氧化物歧化酶 (SOD) 和过氧化氢酶 (CAT) 活性、丙二醛 (MDA) 含量和活性氧 (ROS) 含量与对照组相似或更低。此外，如C. pyrenoidosa的代谢组学分析所示，苯酚和 4-氟苯酚处理组的光合色素和甘油磷脂含量均显着上调，导致与对照组相比藻类细胞生长旺盛。因此，C. pyrenoidosa可以作为苯酚和 4-氟苯酚的优良生物吸附剂。