In nanotechnology, compounds containing metal materials are used in pharmaceutical sciences. The main purpose of this research was to introduce a novel method to control the amount of zeolite imidazolate framework (ZIF) in water by forming a protective layer such as layered double hydroxide (LDH). Firstly, ZIF was synthesised as the nucleus of the nanocomposite, and then LDH was formed by in situ synthesis as a protective layer. Scanning electron microscope, Fourier-transform infrared spectroscopy, X-Ray Diffraction, and Brunauer, Emmett and Teller techniques were used to determine (ZIF-8@LDH chemical structure and morphology. Our findings revealed that the ZIF-8@LDH-MTX complex could interact with carboxyl groups and trivalent cations by creating a bifurcation bridge, clarity, and high thermal stability. The antibacterial test indicated that ZIF-8@LDH was able to inhibit pathogenic growth. 2,5-Diphenyl-2H-Tetrazolium Bromide assay results showed that ZIF-8@LDH alone had no notable cytotoxic effect on Michigan Cancer Foundation-7 (MCF-7) cancer cells. However, the cytotoxicity rate was significantly increased in treated MCF-7 cells with ZIF-8@LDH-MTX compared to that of treated cells with methotrexate alone, which can be reasoned by the protection of drug structure and increasing its permeability. The drug release profile was constant at pH = 7.4. All findings indicated that the ZIF-8@LDH complex could be considered a newly proposed solution for effective anti-cancer drug delivery.

中文翻译：

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沸石咪唑酯骨架-8@层状双氢氧化物多面体纳米复合材料的合成，具有设计的多孔空隙，作为抗癌药物控制递送的有效载体。

在纳米技术中，含有金属材料的化合物用于制药科学。本研究的主要目的是介绍一种通过形成层状双氢氧化物（LDH）等保护层来控制水中沸石咪唑酯骨架（ZIF）含量的新方法。首先合成ZIF作为纳米复合材料的核，然后原位合成形成LDH作为保护层。使用扫描电子显微镜、傅里叶变换红外光谱、X射线衍射以及 Brunauer、Emmett 和 Teller 技术来确定 (ZIF-8@LDH 化学结构和形态。我们的研究结果表明，ZIF-8@LDH-MTX 复合物可以通过形成分叉桥与羧基和三价阳离子相互作用，具有透明度和高热稳定性。抗菌测试表明ZIF-8@LDH能够抑制病原菌的生长。2,5-二苯基-2H-四唑溴化物测定结果表明，单独的 ZIF-8@LDH 对密歇根癌症基金会-7 (MCF-7) 癌细胞没有显着的细胞毒性作用。然而，与单独用甲氨蝶呤处理的细胞相比，ZIF-8@LDH-MTX处理的​​MCF-7细胞的细胞毒性率显着增加，这可能是由于保护了药物结构并增加了其渗透性所致。药物释放曲线在 pH = 7.4 时保持恒定。所有研究结果表明，ZIF-8@LDH 复合物可被视为一种新提出的有效抗癌药物递送解决方案。5-二苯基-2H-四唑溴化物测定结果表明，单独的 ZIF-8@LDH 对密歇根癌症基金会-7 (MCF-7) 癌细胞没有显着的细胞毒性作用。然而，与单独用甲氨蝶呤处理的细胞相比，ZIF-8@LDH-MTX处理的​​MCF-7细胞的细胞毒性率显着增加，这可能是由于保护了药物结构并增加了其渗透性所致。药物释放曲线在 pH = 7.4 时保持恒定。所有研究结果表明，ZIF-8@LDH 复合物可被视为一种新提出的有效抗癌药物递送解决方案。5-二苯基-2H-四唑溴化物测定结果表明，单独的 ZIF-8@LDH 对密歇根癌症基金会-7 (MCF-7) 癌细胞没有显着的细胞毒性作用。然而，与单独用甲氨蝶呤处理的细胞相比，ZIF-8@LDH-MTX处理的​​MCF-7细胞的细胞毒性率显着增加，这可能是由于保护了药物结构并增加了其渗透性所致。药物释放曲线在 pH = 7.4 时保持恒定。所有研究结果表明，ZIF-8@LDH 复合物可被视为一种新提出的有效抗癌药物递送解决方案。这可能是由于保护药物结构、增加其渗透性所致。药物释放曲线在 pH = 7.4 时保持恒定。所有研究结果表明，ZIF-8@LDH 复合物可被视为一种新提出的有效抗癌药物递送解决方案。这可能是由于保护药物结构、增加其渗透性所致。药物释放曲线在 pH = 7.4 时保持恒定。所有研究结果表明，ZIF-8@LDH 复合物可被视为一种新提出的有效抗癌药物递送解决方案。