Purpose

Aedes aegypti mosquito-borne diseases have a significant impact on public health in Brazil. In this study, we investigated the presence of the Zika virus (ZIKV) and dengue virus (DENV) in serum and urine samples from symptomatic participants who attended an Emergency Care Unit located in a city in the northwestern region of São Paulo between February 2018 and April 2019.

Methods

Serum and urine samples were collected from participants suspected of having arbovirus infection. After the extraction of viral RNA, viral detection was performed by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) (One-Step RT-qPCR).

Results

A total of 305 participants participated in this study. A total of 283 blood and 270 urine samples were collected. Of 305 patients, 36.4% (111/305) were positive for ZIKV, 43.3% (132/305) for DENV2, and 0.3% (1/305) for DENV1. Coinfection with ZIKV/DENV2 was observed in 13.1% of participants. If only serum samples were used, ZIKV detection would have decreased to 23.3% (71/305). Of all the participants included in the study, only one was suspected of having ZIKV infection based on clinical diagnosis, and the remaining participants were suspected of having DENV.

Conclusion

By testing serum and urine samples, we increased the detection of both viruses and detected considerable levels of ZIKV and DENV-2 coinfection when compared to other studies. Additionally, we detected an unnoticed ZIKV outbreak in the city. These findings highlight the importance of the molecular diagnosis of arboviruses to aid public health surveillance and management strategies.

中文翻译：

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通过血清和尿液检测检测巴西东南部的 DENV-2 和 ZIKV 双重感染

目的

埃及伊蚊传播的疾病对巴西的公共卫生产生重大影响。在这项研究中，我们调查了 2018 年 2 月至 2018 年 2 月至 2018 年 2 月期间在圣保罗西北部地区城市急诊室就诊的有症状参与者的血清和尿液样本中是否存在寨卡病毒 (ZIKV) 和登革热病毒 (DENV)。 2019 年 4 月。

方法

从疑似虫媒病毒感染的参与者身上采集血清和尿液样本。提取病毒RNA后，通过实时定量逆转录聚合酶链反应（RT-qPCR）（一步RT-qPCR）进行病毒检测。

结果

共有 305 名参与者参与了这项研究。总共收集了283份血液样本和270份尿液样本。在 305 名患者中，36.4% (111/305) 的 ZIKV 呈阳性，43.3% (132/305) 的 DENV2 呈阳性，0.3% (1/305) 的 DENV1 呈阳性。13.1% 的参与者观察到 ZIKV/DENV2 混合感染。如果仅使用血清样本，ZIKV 检测率将下降至 23.3% (71/305)。在该研究纳入的所有参与者中，只有一名根据临床诊断怀疑患有 ZIKV 感染，其余参与者均怀疑患有 DENV。

结论

通过检测血清和尿液样本，我们增加了这两种病毒的检测，与其他研究相比，检测到了相当水平的 ZIKV 和 DENV-2 双重感染。此外，我们还发现该市爆发了未被注意到的寨卡病毒疫情。这些发现强调了虫媒病毒分子诊断对于帮助公共卫生监测和管理策略的重要性。