ABSTRACT

The study aimed to investigate the effect of fermented n-hexane extract of Pentaclethra macrophylla seeds (FPMSE) in an experimental model of glaucoma. Eighteen rabbits (1-1.8 kg body weight) were allocated into six groups of three rabbits (six eyes per group). Group 1 was the normal control. Ocular hypertension was induced with 1% prednisolone acetate ophthalmic solution in groups 2-6, twice daily for 21 days. Group 2 received no treatment, while groups 3–6 received 5 mg/kg b.w. acetazolamide, 100, 200, and 300 mg/kg b.w. extract, respectively. The intraocular pressures and other biochemical parameters were evaluated. The existence of bioactive compounds was determined via phytochemical analysis. Compared to group 2, the administration of FPMSE resulted in a significant decrease in IOP in the treated groups. Malondialdehyde concentrations in the treated groups’ aqueous humor and plasma were considerably lower (p < 0.05) than in group 2. Compared to group 2, glutathione, nitric oxide, and antioxidant levels improved significantly (p < 0.05). C-reactive protein and glutamate levels in group 2 were comparable (p > 0.05). FPMSE substantially reduced phospholipase A₂ activity. Histological assessment of the ciliary body showed adequate pigmentations in the treatment groups compared to group 2. Hence, FPMSE possesses ocular hypotensive effects.

摘要

本研究旨在探讨五叶草发酵正己烷提取物的作用青光眼实验模型中的种子 (FPMSE)。将十八只兔子（体重1-1.8公斤）分成六组，每组三只（每组六只眼）。第 1 组为正常对照。2~6组给予1%醋酸泼尼松龙眼液诱导高眼压，每日2次，共21天。第 2 组未接受任何治疗，而第 3-6 组分别接受 5 mg/kg bw 乙酰唑胺、100、200 和 300 mg/kg bw 提取物。评估眼内压和其他生化参数。通过植物化学分析确定生物活性化合物的存在。与第 2 组相比，FPMSE 的施用导致治疗组的 IOP 显着降低。治疗组房水和血浆中的丙二醛浓度显着降低（p < 0.05) 低于第 2 组。与第 2 组相比，谷胱甘肽、一氧化氮和抗氧化剂水平显着提高 ( p  < 0.05)。第 2 组的 C 反应蛋白和谷氨酸水平具有可比性 ( p  > 0.05)。FPMSE 显着降低了磷脂酶 A ₂的活性。与第 2 组相比，睫状体的组织学评估显示治疗组有足够的色素沉着。因此，FPMSE 具有降眼压作用。