This study was conducted to determine the possible effects of intracerebroventricular MOTS-c infusion on thyroid hormones and uncoupling proteins (UCPs) in rats. Forty male Wistar Albino rats were divided into 4 groups with 10 animals in each group: control, sham, 10 and 100 µM MOTS-c. Hypothalamus, blood, muscle, adipose tissues samples were collected for thyrotropin-releasing hormone (TRH), UCP1 and UCP3 levels were determined by the RT-PCR and western blot analysis. Serum thyroid hormone levels were determined by the ELISA assays. MOTS-c infusion was found to increase food consumption but it did not cause any changes in the body weight. MOTS-c decreased serum TSH, T3, and T4 hormone levels. On the other hand, it was also found that MOTS-c administration increased UCP1 and UCP3 levels in peripheral tissues. The findings obtained in the study show that central MOTS-c infusion is a directly effective agent in energy metabolism.

本研究旨在确定脑室内 MOTS-c 输注对大鼠甲状腺激素和解偶联蛋白 (UCP) 的可能影响。将 40 只雄性 Wistar Albino 大鼠分为 4 组，每组 10 只：对照组、假手术组、10 和 100 µM MOTS-c。收集下丘脑、血液、肌肉、脂肪组织样本进行促甲状腺素释放激素(TRH)、RT-PCR和蛋白质印迹分析测定UCP1和UCP3水平。通过 ELISA 测定测定血清甲状腺激素水平。研究发现，MOTS-c 输注会增加食物消耗，但不会导致体重发生任何变化。MOTS-c 降低血清 TSH、T3 和 T4 激素水平。另一方面，还发现MOTS-c给药增加了外周组织中的UCP1和UCP3水平。研究结果表明，中枢 MOTS-c 输注是能量代谢的直接有效药物。