We developed microsatellite markers to use in studying the population genetics of the trematode Alloglossidium renale, a fluke with a precocious life cycle where sexual maturation occurs in a grass shrimp. Among 21 tested loci in a Mississippi population sample, 14 were polymorphic, 12 of which significantly deviated from Hardy-Weinberg Equilibrium (HWE). We estimated identity disequilibrium (ID) to confirm whether the deviations from HWE were due to significant amounts of selfing or due to technical factors. The selfing rate derived from F_IS was 86.6%, whereas the selfing rate obtained by ID was 83.9%, indicating that the deviation in HWE was due to a high amount of selfing within the population. These markers will be useful for ecological and evolutionary studies of A. renale especially in relation to the interplay of hermaphroditic mating systems, inbreeding depression, and transmission dynamics.

我们开发了微卫星标记，用于研究吸虫Alloglossidium renale的种群遗传学，这是一种具有早熟生命周期的吸虫，在草虾中发生性成熟。在密西西比人口样本的 21 个测试基因座中，14 个是多态性的，其中 12 个显着偏离 Hardy-Weinberg 平衡 (HWE)。我们估计身份不平衡 (ID) 以确认与 HWE 的偏差是由于大量的自交还是由于技术因素。来自F _{IS的自交率}为 86.6%，而 ID 获得的自交率为 83.9%，表明 HWE 的偏差是由于种群内大量自交造成的。这些标记将有助于A. renale的生态学和进化研究，尤其是与雌雄同体交配系统、近亲繁殖抑制和传播动力学的相互作用有关的研究。