Abstract

In this study, fungal strains designated as KNUF-22-14A and KNUF-22-15A were isolated from soil samples in Korea. These two strains were identified based on cultural and morphological characteristics as well as phylogenetic analyses and were found to be morphologically and phylogenetically identical. Upon their morphological comparison with closely related species, such as Tolypocladium album, T. amazonense, T. endophyticum, T. pustulatum, and T. tropicale, a difference in the size of short phialides [0.6–2.4(–9.3) × 0.8–1.4 µm] was observed. Meanwhile, these strains had larger conidia (1.2–3.0 × 1.2–3.0 µm) than T. album, T. amazonense, T. endophyticum, and T. tropicale and smaller conidia than T. pustulatum. Phylogenetic analyses using a multi-locus datasets based on ITS, LSU, and SSU showed that KNUF-22-14A and KNUF-22-15A formed a distinct cluster from previously identified Tolypocladium species. Thus, these fungal strains isolated from soil in Korea are proposed as a novel species according to their characteristics and are named Tolypocladium globosum sp. nov.

摘要

在这项研究中，指定为 KNUF-22-14A 和 KNUF-22-15A 的真菌菌株是从韩国的土壤样本中分离出来的。这两个菌株是根据培养和形态学特征以及系统发育分析鉴定的，发现它们在形态学和系统发育学上是相同的。在与密切相关的物种（例如Tolypocladium album、T. amazonense、T. endophyticum、T. pustulatum和T. tropicale ）进行形态学比较后，短瓶梗的大小差异 [0.6–2.4(–9.3) × 0.8– 1.4 µm] 被观察到。同时，这些菌株的分生孢子 (1.2–3.0 × 1.2–3.0 µm) 比T. album、T. amazonense、T. endophyticum和T. tropicale以及比T. pustulatum更小的分生孢子。使用基于 ITS、LSU 和 SSU 的多位点数据集进行的系统发育分析表明，KNUF-22-14A 和 KNUF-22-15A 形成了一个与之前鉴定的Tolypocladium物种不同的簇。因此，这些从韩国土壤中分离出来的真菌菌株根据它们的特性被提议作为一个新物种，并被命名为Tolypocladium globosum sp。十一月