Abstract

We investigated antifungal properties of two kraft-pulping liquors as potential environmentally friendly wood preservatives targeting in deterioration by white rot (WRF) and brown rot fungi (BRF). The in vitro antifungal activity of crude black liquor (BL) and the product from a hydrothermal gasification (BLW_Supc) process were investigated. Both products showed high bioactivity, with BL completely inhibiting the activity of WRF and BRF; 80% was entirely suppressed at low BL concentrations (5–10% vol/vol). Using the BLW_Supc at 2.55% wt/wt, we achieved the same inhibitory effect as BL (15% vol/vol) for the two fungi. These antifungal effects can be explained by the composition of liquors that act as antioxidants, promoting enzymatic inactivation and inhibiting the growth of WRF and BRF. BLW_Supc exhibited radical quenching activity against DPPH of 43.9%, and the BL, a phenolic content about 20 times higher (23.635 mg/L) than BLW_Supc (2.093 mg/L), suggesting that antifungal activity of BL and BLW is related to their chemical composition. An opportunity demonstrated by this study is the reuse of BLW_Supc as a protective treatment within a sustainable chain.

摘要

我们研究了两种硫酸盐制浆液的抗真菌特性，作为潜在的环境友好型木材防腐剂，旨在防止白腐病 (WRF) 和褐腐病真菌 (BRF) 造成的劣化。研究了粗黑液 (BL) 和水热气化 (BLW _{Supc ) 工艺产物的}体外抗真菌活性。两种产品均表现出较高的生物活性，BL完全抑制WRF和BRF的活性；80% 在低 BL 浓度（5–10% vol/vol）下被完全抑制。使用 BLW _SPC在 2.55% wt/wt 时，我们对两种真菌实现了与 BL (15% vol/vol) 相同的抑制效果。这些抗真菌作用可以通过充当抗氧化剂的液体成分来解释，促进酶促失活并抑制 WRF 和 BRF 的生长。BLW _Supc对 DPPH 的自由基猝灭活性为 43.9%，而 BL 的酚类含量 (23.635 mg/L) 比 BLW Supc ( _2.093 mg/L) 高约 20 倍，表明 BL 和 BLW 的抗真菌活性与它们的化学成分。本研究展示的一个机会是将 BLW _Supc作为可持续链中的保护性处理进行再利用。