Crossbreeding is the most commonly used method in breeding of Lentinula edodes, however low fruiting rate of the hybrids has always caused troubles and barriers for breeders. An early screening method of the fruiting ability could make the breeding work more efficient. In this paper, a rapid and high-throughput laccase activity detection method based on agar diffusion principle was developed. In this way, we investigated the constitutive and inducible extracellular laccase activity of 36 strains in a breeding population of L. edodes on different media and performed a correlation analysis with fruiting ability of these strains. The results showed the laccase activity of mycelium cultured in non-induced medium for 8 d could be used as an early screening index to judge whether it had fruiting ability at the later stage. Early rapid and simple screening method for hybrid populations was established based on laccase activity characteristics of mycelia. 127 strains from another 5 different hybrid populations were used to verify the early screening method. From the validation results, the early screening method was effective, but the appropriate screening threshold was needed to select according to the cross population, which would greatly to improve the breeding efficiency of L. edodes.

中文翻译：

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香菇杂交后代结实能力早期筛选技术研究

杂交育种是香菇育种中最常用的方法，但杂交后代结果率低一直是育种者的困扰和障碍。一种出菇能力的早期筛选方法可以使育种工作更有效率。本文开发了一种基于琼脂扩散原理的快速、高通量的漆酶活性检测方法。通过这种方式，我们研究了香菇繁殖群体中 36 个菌株在不同培养基上的组成型和诱导型胞外漆酶活性，并与这些菌株的结果能力进行了相关性分析。结果表明，在非诱导培养基中培养8 d的菌丝体漆酶活性可作为判断其后期是否具有出菇能力的早期筛选指标。建立了基于菌丝体漆酶活性特征的杂交种群早期快速简便筛选方法。来自另外5个不同杂交种群的127个菌株用于验证早期筛选方法。从验证结果来看，早期筛选方法是有效的，但需要根据杂交群体选择合适的筛选阈值，这将大大提高香菇的育种效率。