High podoplanin (PDPN) expression correlates with poor prognosis in various cancers. However, the expression and clinical value of PDPN in glioma are unclear. In this study, PDPN expression was compared in 227 glioma tissues and 22 paired non-neoplastic tissues, and its association with prognostic factors was statistically analyzed. The effect of PDPN knockdown on the proliferation ability of glioma cells (U87MG and U118MG cell lines) was assessed along with the underlying molecular mechanism. Overexpression of PDPN was observed in the majority of glioma tissues compared with the expression in normal tissues. PDPN overexpression was positively correlated with IDH wild-type status, TERT promoter mutation status, and ATRX retention status, and was negatively correlated with 1p/19q codeletion status. The expression level of PDPN was positively correlated with the glioma grade in the diffuse astrocytoma, IDH wild-type. High PDPN expression was also negatively correlated with survival in astrocytoma patients with IDH mutation or wild-type and in glioblastoma patients with IDH wild-type. Grade, radiochemotherapy, and PDPN overexpression emerged as independent indicators for a poor prognosis of glioma patients. PDPN knockdown suppressed proliferation and reduced p-Akt and p-mTOR protein expression in glioma cells. PDPN is a potential biomarker or therapeutic target for glioma that is closely associated with tumor grade and poor prognosis, which may play a role in enhancing cell proliferation via Akt/mTOR signaling.

中文翻译：

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Podoplanin 的过度表达预示着神经胶质瘤患者的不良预后。

平足蛋白 (PDPN) 高表达与各种癌症的不良预后相关。然而，PDPN在胶质瘤中的表达和临床价值尚不清楚。本研究比较了 227 例胶质瘤组织和 22 配对非肿瘤组织中的 PDPN 表达，并对其与预后因素的关系进行了统计分析。评估 PDPN 敲低对神经胶质瘤细胞（U87MG 和 U118MG 细胞系）增殖能力的影响及其潜在分子机制。与正常组织中的表达相比，在大多数胶质瘤组织中观察到 PDPN 过度表达。PDPN过表达与IDH野生型状态、TERT启动子突变状态和ATRX保留状态呈正相关，与1p/19q共缺失状态呈负相关。在弥漫性星形细胞瘤IDH野生型中，PDPN的表达水平与胶质瘤分级呈正相关。PDPN 高表达也与 IDH 突变或野生型星形细胞瘤患者和 IDH 野生型胶质母细胞瘤患者的生存呈负相关。分级、放化疗和 PDPN 过度表达成为胶质瘤患者预后不良的独立指标。PDPN 敲低可抑制胶质瘤细胞的增殖并减少 p-Akt 和 p-mTOR 蛋白表达。PDPN是胶质瘤的潜在生物标志物或治疗靶点，与肿瘤分级和不良预后密切相关，可能通过Akt/mTOR信号传导增强细胞增殖。