Aflatoxins (AF) are fungal toxins which not only contaminate food, but also adversely impact human health if consumed and serve as biomarkers for deadly diseases like liver cancer. Therefore, there is a strong need to develop ultra-sensitive AF detection method in physiological environments. In the present study, ternary transition metal sulfides single crystals (Mn_0.02Ta₃S₆ and Fe₀.₆₅Ta₃S₆) were grown, exfoliated to thin layered nanosheets (NSs) followed by polyethylene-glycol modification (PEG@NSs). High fluorescence quenching abilities towards the aptamers for AF biosensing were demonstrated using PEGylated NSs. It has been observed that PEGylated NSs improves the detection sensitivity ∼ 248 times better than non-PEGylated NSs. Subsequently, multiplexed detections of aflatoxin B1 (AFB1) and M1 (AFM1) in diverse mediums including PBS buffer (1 ×), milk and human serum were performed by employing PEG@Mn₀.₀₂Ta₃S₆ NSs with TAMRA dye-labelled AFB1 aptamer and FAM dye-labelled AFM1 aptamer, respectively. The fluorescence intensity of designed sensor exhibited ultrasensitive detections (∼ pM) and a wide linear range (≥ 5 orders). Hence, the present study can serve as a unique platform for facile, ultra-sensitive, selective, cost-effective and quick multiplexed detection of food toxins and disease biomarkers in complex-matrix.

黄曲霉毒素 (AF) 是一种真菌毒素，它不仅会污染食物，如果被食用还会对人类健康产生不利影响，并可作为肝癌等致命疾病的生物标志物。因此，迫切需要在生理环境中开发超灵敏的自动对焦检测方法。在本研究中，三元过渡金属硫化物单晶（Mn _0.02 Ta ₃ S ₆和 Fe ₀ . ₆₅ Ta ₃ S ₆) 生长，剥离成薄层纳米片 (NSs)，然后进行聚乙二醇改性 (PEG@NSs)。使用聚乙二醇化的 NSs 证明了对用于 AF 生物传感的适体的高荧光猝灭能力。已经观察到聚乙二醇化的 NSs 比非聚乙二醇化的 NSs 提高了检测灵敏度～248 倍。随后，通过使用 PEG@Mn 0 对包括 PBS 缓冲液 (1 ×)、牛奶和人血清在内的多种介质中的黄曲霉毒素 B1 (AFB1) 和 M1 (AFM1) 进行多重_检测。₀₂ Ta ₃ S ₆NSs 分别带有 TAMRA 染料标记的 AFB1 适体和 FAM 染料标记的 AFM1 适体。所设计传感器的荧光强度表现出超灵敏检测 (∼ pM) 和宽线性范围 (≥ 5 个数量级)。因此，本研究可以作为一个独特的平台，用于对复杂基质中的食物毒素和疾病生物标志物进行简便、超灵敏、选择性、成本效益和快速多重检测。