Abstract

Aim

To determine the optimum condition for preparing chitooligosaccharide-catechin conjugate (COS-CAT) liposomes using different stabilising agents.

Methods

COS-CAT liposomes (0.1–1%, w/v) were prepared using soy phosphatidylcholine (SPC) (50–200 mM) and glycerol or cholesterol (25–100 mg). Encapsulation efficiency (EE), loading capacity (LC), physicochemical characteristics, FTIR spectra, thermal stability, and structure of COS-CAT liposomes were assessed.

Results

COS-CAT loaded liposome stabilised by cholesterol (COS-CAT-CHO) showed higher stability as shown by the highest EE (76.81%) and LC (4.57%) and the lowest zeta potential (ZP) (−76.51 mV), polydispersity index (PDI) (0.2674) and releasing efficiency (RE) (53.54%) (p < 0.05). COS-CAT-CHO showed the highest retention and relative remaining bioactivities of COS-CAT under various conditions (p < 0.05). FTIR spectra revealed the interaction between the choline group of SPC and -OH groups of COS-CAT. Phase transition temperature of COS-CAT-CHO was shifted to 184 °C, which was higher than others (p < 0.05).

Conclusion

SPC and cholesterol-based liposome could be used as a promising vesicle for maintaining bioactivities of COS-CAT.

中文翻译：

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使用不同稳定剂的壳寡糖-儿茶素缀合物负载脂质体：特性、稳定性和生物活性

摘要

目的

确定不同稳定剂制备壳寡糖-儿茶素缀合物（COS-CAT）脂质体的最佳条件。

方法

使用大豆磷脂酰胆碱 (SPC) (50–200 mM) 和甘油或胆固醇 (25–100 mg) 制备 COS-CAT 脂质体 (0.1–1%, w/v)。评估了 COS-CAT 脂质体的包封效率 (EE)、负载能力 (LC)、理化特性、FTIR 光谱、热稳定性和结构。

结果

胆固醇稳定的 COS-CAT 负载脂质体 (COS-CAT-CHO) 显示出更高的稳定性，最高的 EE (76.81%) 和 LC (4.57%) 以及最低的 zeta 电位 (ZP) (−76.51 mV)、多分散指数(PDI) (0.2674) 和释放效率 (RE) (53.54%) ( p  < 0.05)。COS-CAT-CHO 在各种条件下表现出 COS-CAT 的最高保留率和相对剩余生物活性 ( p  < 0.05)。FTIR 光谱揭示了 SPC 的胆碱基团和 COS-CAT 的 -OH 基团之间的相互作用。COS-CAT-CHO 的相变温度移至 184 °C，高于其他材料 ( p  < 0.05)。

结论

SPC 和胆固醇脂质体可用作维持 COS-CAT 生物活性的有前途的囊泡。