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Illumina MiSeq sequencing investigation on the contrasting rhizosphere soil bacterial community structures in tea orchard soil under different content of aluminium
Frontiers in Life Science ( IF 1.333 ) Pub Date : 2023-05-02 , DOI: 10.1080/26895293.2023.2206544
Yunfei Hu 1 , Huan Li 2 , Yang Zhou 1 , Shuilian Gao 1 , Rajiv Periakaruppan 3, 4 , Huiling Mei 5 , Jianjie Li 5 , Xiao Yuan 3 , Xiaobing Kou 3 , Xinghui Li 3 , Xuan Chen 3
Affiliation  

The rhizosphere of grown tea (Camellia sinensis (L.) O. Kuntze) becomes acidic, and aluminium (Al) toxicity has been identified as a key constraint on plant development in acidic soils. The current study investigated the composition and functions of rhizosphere bacteria as well as soil enzyme activity under different Al stress conditions. The effect of soil pH, soil enzyme activities, the diversity and structure of the rhizosphere bacterial population under various Al concentrations were studied by the pot experiment. All analyses demonstrated that substantial alterations in the activity of soil enzymes and the composition of the bacterial communities in the rhizosphere could only be seen under conditions of extreme Al stress. Soil enzymes were more concentrated in Al concentrations of 100, 150 and 250 µM, respectively. More than 82,000 valid reads were obtained for each replicate, and the abundance of certain genera in the aluminium treated groups were greater than the control group. Lower Al stress attracted Al-tolerant bacteria such as Burkholderia to increase Al stress resistance. These findings revealed that soil enzyme activities and the structure of the rhizosphere bacterial population in tea orchard soil provided tolerance or resistance capability to tea plants for their development under Al stress.



中文翻译:

不同铝含量下茶园土壤根际土壤细菌群落结构对比Illumina MiSeq测序研究

种植茶叶的根际(Camellia sinensis(L.) O. Kuntze) 变成酸性,铝 (Al) 毒性已被确定为酸性土壤中植物发育的关键制约因素。目前的研究调查了不同铝胁迫条件下根际细菌的组成和功能以及土壤酶活性。通过盆栽试验研究了不同Al浓度下土壤pH值、土壤酶活性、根际细菌种群多样性和结构的影响。所有分析表明,土壤酶活性和根际细菌群落组成的显着变化只能在极端铝胁迫条件下才能看到。土壤酶分别更集中于 100、150 和 250 µM 的铝浓度。每个重复获得超过 82,000 个有效读数,并且铝处理组中某些属的丰度大于对照组。较低的铝胁迫吸引耐铝细菌,如伯克霍尔德氏菌,以增加铝胁迫抗性。这些发现表明,茶园土壤中的土壤酶活性和根际细菌种群结构为茶树在铝胁迫下的发育提供了耐受性或抗性能力。

更新日期:2023-05-03
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