Abstract

Abuse of dopings is one of the serious issues affecting competitive sports globally. Traditional means of analysis for doping detection face the challenges of costly, bulky, and complicated pretreatment, as well as long detection times. Therefore, it is critical to achieve on-site and rapid detection for dopings. Herein, we proposed a strategy for ultrasensitive and quick detection of multiple dopings using a combination of liquid phase extraction and surface-enhanced Raman spectroscopy. The clenbuterol, methadone, oxycodone, and chlordiazepoxide were four dopings, which could be detected conveniently in human saliva and urine using the above approach. The limit of detection for clenbuterol, methadone, oxycodone, and chlordiazepoxide in saliva were 25, 10, 50, and 25 ng/mL, respectively, and in urine, 25, 5, 50, and 25 ng/mL. Finally, in about a minute, four dopings at concentration of 100 ng/mL in saliva and urine can be detected accurately and synchronously. This synchronous detection strategy further demonstrates the enormous advantages of SERS in on-site and rapid detection of multiple drugs.

摘要

滥用兴奋剂是影响全球竞技体育的严重问题之一。传统的兴奋剂检测分析手段面临成本高、体积大、预处理复杂、检测时间长等挑战。因此，实现兴奋剂的现场快速检测至关重要。在此，我们提出了一种使用液相萃取和表面增强拉曼光谱相结合的超灵敏和快速检测多种掺杂的策略。盐酸克伦特罗、美沙酮、羟考酮和利眠宁是四种兴奋剂，采用上述方法可以方便地在人体唾液和尿液中进行检测。唾液中克伦特罗、美沙酮、羟考酮和利眠宁的检测限分别为 25、10、50 和 25 ng/mL，尿液中的检测限分别为 25、5、50 和 25 ng/mL。最后，在大约一分钟内，唾液和尿液中浓度为100 ng/mL的四种兴奋剂可以同时准确检测出来。这种同步检测策略进一步证明了SERS在多种药物现场快速检测方面的巨大优势。