Background: Despite the crucial involvement of vitronectin in affecting the perseverance of certain respiratory pathogens and the progression of several lung diseases, the association of vitronectin with tuberculosis (TB) has been poorly studied. The present study aimed to determine whether vitronectin levels are altered in TB patients compared to healthy humans. Methods: Twenty-four laboratory-confirmed tuberculosis patients (pulmonary TB -8, extrapulmonary- 8 and HIV-TB dual infected -8) and eight healthy individuals were included in this study. The quantitative detection of vitronectin in serum-derived exosomes of study participants was performed using a sandwich enzyme-linked immunosorbent assay. Measured concentrations of vitronectin were compared with the demographic variables of the study participants and between the study groups. The Mann–Whitney U unpaired test was used in statistical analysis, and the p-value < 0.05 was considered statistically significant. Results: Vitronectin was detected in serum-derived exosomes of all study participants. The demographic characteristics (gender, age, smoking and alcohol consumption habit, history of cough, and weight loss) were not significantly correlated with the vitronectin concentrations of the study participants (p-value> 0.05). The level of vitronectin was higher in patients with pulmonary TB (778.54 ng/l) and extra-pulmonary-TB patients (773.04 ng/l) while lower in HIV-pulmonary TB dual-infected patients (354.86 ng/l) as compared to healthy humans (456.20ng/l). There was a significant difference between vitronectin concentrations of patients with pulmonary TB (p-value: 0.0002) and extrapulmonary TB (p-value: 0.003) compared to healthy controls. Conclusion: The present study reported an increased concentration of vitronectin in serum-derived exosomes of pulmonary and extra-pulmonary TB patients compared to HIV-TB dual-infected patients and healthy humans. Further studies are needed to fully elucidate the diagnostic potential and functionalities of higher concentrations of vitronectin in the pathogenic processes of human TB.

中文翻译：

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与 HIV-结核双重感染患者和健康人相比，肺结核和肺外结核患者血清来源的外泌体中玻连蛋白（S 蛋白）的丰度更高

背景：尽管玻连蛋白在影响某些呼吸道病原体的持续存在和几种肺部疾病的进展方面起着至关重要的作用，但玻连蛋白与结核病 (TB) 的关联研究很少。本研究旨在确定与健康人相比，结核病患者的玻连蛋白水平是否发生了变化。方法：本研究纳入了 24 名实验室确诊的结核病患者（肺结核 -8、肺外结核 -8 和 HIV-TB 双重感染 -8）和 8 名健康个体。使用夹心酶联免疫吸附试验对研究参与者血清来源的外泌体中的玻连蛋白进行定量检测。将测量的玻连蛋白浓度与研究参与者和研究组之间的人口统计变量进行比较。Mann-Whitney U 未配对检验用于统计分析，p 值 < 0.05 被认为具有统计学意义。结果：在所有研究参与者的血清来源的外泌体中检测到玻连蛋白。人口统计学特征（性别、年龄、吸烟和饮酒习惯、咳嗽史和体重减轻）与研究参与者的玻连蛋白浓度无显着相关性（p 值 > 0.05）。肺结核患者 (778.54 ng/l) 和肺外结核患者 (773.04 ng/l) 的玻连蛋白水平较高，而 HIV-肺结核双重感染患者 (354.86 ng/l)健康人 (456.20ng/l)。肺结核患者（p 值：0.0002）和肺外结核患者（p 值：0.0002）的玻连蛋白浓度之间存在显着差异。003) 与健康对照相比。结论：本研究报道，与 HIV-TB 双重感染患者和健康人相比，肺结核和肺外结核患者血清来源的外泌体中玻连蛋白浓度增加。需要进一步的研究来充分阐明高浓度玻连蛋白在人类结核病致病过程中的诊断潜力和功能。