Abstract

The fungal strain KNUF-22-18B, belonging to Cucurbitariaceae, was discovered from a stink bug (Hygia lativentris) during the investigation of insect microbiota in Chungnam Province, South Korea. The colonies of the strain KNUF-22-18B were wooly floccose, white to brown in the center on oatmeal agar (OA), and the colonies were buff, margin even, and colorless, reverse white to yellowish toward the center on malt extract agar (MEA). The strain KNUF-22-18B produced pycnidia after 60 days of culturing on potato dextrose agar, but pycnidia were not observed on OA. On the contrary, N. keratinophila CBS 121759^T abundantly formed superficial pycnidia on OA and MEA after a few days. The strain KNUF-22-18B produced chlamydospores subglobose to globose, mainly in the chain, with a small diameter of 4.4–8.8 μm. At the same time, N. keratinophila CBS 121759^T displayed a globose terminal with a diameter of 8–10 μm. A multilocus phylogeny using the internal transcribed spacer regions, 28S rDNA large subunit, β-tubulin, and RNA polymerase II large subunit genes further validated the uniqueness of the strain. The detailed description and illustration of the proposed species as Neocucurbitaria chlamydospora sp. nov. from Korea was strongly supported by molecular phylogeny.

摘要

真菌菌株KNUF-22-18B属于葫芦科，是在韩国忠清南道调查昆虫微生物群时从臭虫（Hygia lativentris ）中发现的。菌株KNUF-22-18B在燕麦琼脂（OA）上菌落呈毛状絮状，中心白色至棕色；在麦芽提取物琼脂上菌落呈浅黄色，边缘均匀，无色，向中心反白至淡黄色（MEA）。菌株KNUF-22-18B在马铃薯葡萄糖琼脂上培养60天后产生分生孢子器，但在OA上未观察到分生孢子器。相反，N. keratinophila CBS 121759 ^T几天后，OA和MEA上大量形成浅表分生孢子器。菌株KNUF-22-18B产生厚垣孢子，近球形至球形，以链状为主，直径较小，为4.4～8.8μm。同时，N. keratinophila CBS 121759 ^T显示出直径为 8-10 μm 的球形末端。使用内部转录间隔区、28S rDNA 大亚基、β-微管蛋白和 RNA 聚合酶 II 大亚基基因的多位点系统发育进一步验证了该菌株的独特性。对拟议物种Neocucurbitaria chlamydospora sp. 的详细描述和说明。十一月 来自韩国的研究得到了分子系统发育学的大力支持。