Random mutagenesis is a useful tool for identifying genes of interest. Here, we used long-read whole-genome sequencing analysis to identify T-DNA insertion sites in pathogenicity mutants of Colletotrichum higginsianum, a fungus that causes anthracnose on Brassica plants. We identified mutated regions by aligning the sequence reads containing T-DNA to the C. higginsianum reference genome and also detected unexpectedly large chromosomal rearrangements that appear to have been caused by T-DNA transformation. These results demonstrate that long-read whole-genome sequencing is effective for identifying mutated regions in the C. higginsianum mutants.

随机诱变是识别感兴趣基因的有用工具。在这里，我们使用长读长全基因组测序分析来识别Colletotrichum higginsianum致病性突变体中的 T-DNA 插入位点，Colletotrichum higginsianum 是一种导致芸苔属植物炭疽病的真菌。我们通过将包含 T-DNA 的序列读数与C. higginsianum参考基因组比对来识别突变区域，并且还检测到意外的大染色体重排，这些重排似乎是由 T-DNA 转化引起的。这些结果表明，长读长全基因组测序可有效识别C. higginsianum突变体中的突变区域。