The treatment of periodontitis focuses on controlling the progression of inflammation, reducing plaque accumulation, and promoting bone tissue reconstruction. Among them, the reconstruction of irregular bone resorption caused by periodontitis is a long-standing challenge. At present, the local drug treatment of periodontitis is mainly anti-inflammatory and antibacterial drugs. In this study, psoralen (Pso), a Chinese herbal medicine with anti-inflammatory, antibacterial, and osteogenic effects, was selected for the local treatment of periodontitis. Meanwhile, an injectable methacrylate gelatin (GelMA) platform loading with Pso was constructed. Pso-GelMA had the properties of fluidity, light cohesion, self-healing, and slow release, which could be better used in the deep and narrow structure of the periodontal pocket, and greatly increased the effectiveness of local drug delivery. The pore size of Gelma hydrogel did not change after loading Pso by SEM. In vitro, Pso-GelMA effectively upregulated the expression of osteogenic genes and proteins, increased alkaline phosphatase activity, promoted the mineralisation of rat bone marrow mesenchymal stem cells (BMSCs) extracellular matrix, and had significant antibacterial effects on Staphylococcus aureus and Fusobacterium nucleatum. Therefore, Pso-GelMA has immense promise in the adjuvant treatment of periodontitis.

中文翻译：

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含有补骨脂素的可注射甲基丙烯酰化明胶促进成骨及其抑菌特性。

牙周炎的治疗重点是控制炎症进展、减少牙菌斑堆积、促进骨组织重建。其中，牙周炎引起的不规则骨吸收的重建是一个长期存在的挑战。目前，牙周炎的局部药物治疗主要是抗炎和抗菌药物。本研究选择补骨脂素（Pso）这种具有抗炎、抗菌、成骨作用的中草药用于牙周炎的局部治疗。同时构建了负载Pso的可注射甲基丙烯酸明胶（GelMA）平台。Pso-GelMA具有流动性、轻内聚性、自愈性、缓释性等特性，可以更好地应用于牙周袋深部狭窄结构，并大大提高了局部给药的有效性。通过SEM观察负载Pso后Gelma水凝胶的孔径没有变化。在体外，Pso-GelMA有效上调成骨基因和蛋白的表达，增加碱性磷酸酶活性，促进大鼠骨髓间充质干细胞（BMSCs）细胞外基质矿化，并对金黄色葡萄球菌和具核梭杆菌具有显着的抗菌作用。因此，Pso-GelMA在牙周炎的辅助治疗中具有巨大的前景。促进大鼠骨髓间充质干细胞（BMSCs）细胞外基质矿化，对金黄色葡萄球菌、具核梭杆菌有显着抗菌作用。因此，Pso-GelMA在牙周炎的辅助治疗中具有巨大的前景。促进大鼠骨髓间充质干细胞（BMSCs）细胞外基质矿化，对金黄色葡萄球菌、具核梭杆菌有显着抗菌作用。因此，Pso-GelMA在牙周炎的辅助治疗中具有巨大的前景。