ABSTRACT

Aims

Hemophore-like proteins sequester heme from host hemoproteins. We aimed to determine whether the host immune system can recognize not only Porphyromonas gingivalis HmuY but also its homologs expressed by other periodontopathogens, and how periodontitis influences the production of respective antibodies.

Methods

The reactivity of total bacterial antigens and purified proteins with serum IgG antibodies of 18 individuals with periodontitis and 17 individuals without periodontitis was examined by enzyme-linked immunosorbent assay (ELISA). To compare IgG reactivity between groups with and without periodontitis and within the various dilutions of sera, statistical analysis was performed using the Mann-Whitney U-test and two-way ANOVA test with the post-hoc Bonferroni test.

Results

Individuals with periodontitis produced IgG antibodies reacting more strongly not only with total P. gingivalis antigens (P = 0.0002; 1:400) and P. gingivalis HmuY (P = 0.0016; 1:100) but also with Prevotella intermedia PinA (P = 0.0059; 1:100), and with low efficiency with P. intermedia PinO (P = 0.0021; 1:100). No increase in the reactivity of IgG antibodies with Tannerella forsythia Tfo and P. gingivalis HusA was found in individuals with periodontitis.

Conclusions

Although hemophore-like proteins are structurally related, they are differentially recognized by the host immune system. Our findings point to specific antigens, mainly P. gingivalis HmuY and P. intermedia PinA, whose immunoreactivity could be further investigated to develop markers of periodontitis.

中文翻译：

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牙周病原菌产生的血细胞样蛋白被宿主免疫系统识别，并与 IgG 抗体发生不同的反应

摘要

目标

血红素样蛋白将血红素与宿主血红素蛋白隔离。我们的目的是确定宿主免疫系统是否不仅可以识别牙龈卟啉单胞菌HmuY，还可以识别其他牙周病原体表达的其同源物，以及牙周炎如何影响相应抗体的产生。

方法

采用酶联免疫吸附试验（ELISA）检测18例牙周炎患者和17例非牙周炎患者的总细菌抗原和纯化蛋白与血清IgG抗体的反应性。为了比较患有和不患有牙周炎的组之间以及不同稀释度的血清中的 IgG 反应性，使用 Mann-Whitney U 检验和双向 ANOVA 检验以及事后 Bonferroni 检验进行统计分析。

结果

患有牙周炎的个体产生的 IgG 抗体不仅与牙龈卟啉单胞 菌总抗原 ( P  = 0.0002; 1:400) 和牙龈卟啉单胞菌HmuY ( P  = 0.0016; 1:100) 反应更强烈，而且与中间普氏菌PinA ( P  = 0.0059 ) 反应更强烈; 1:100)，而 P. intermedia PinO 的效率较低 ( P  = 0.0021; 1:100)。在患有牙周炎的个体中，未发现 IgG 抗体与连翘坦纳菌Tfo 和牙龈卟啉单胞菌HusA的反应性增加。

结论

尽管血细胞样蛋白在结构上相关，但宿主免疫系统对它们的识别存在差异。我们的研究结果指向特定抗原，主要是牙龈 卟啉单胞菌HmuY 和中间牙龈卟啉单胞 菌 PinA，可以进一步研究其免疫反应性以开发牙周炎标记物。