Ras-related protein Rab-10 (RAB10) is involved in tumorigenesis and progression of hepatocellular carcinoma (HCC). Here, we found RAB10, O-GlcNAc transferase (OGT), and O-GlcNAcylation were upregulated in HCC. In addition, RAB10 protein level was prominently positively correlated with the expression of OGT. O-GlcNAcylation modification of RAB10 was then investigated. Here we showed that RAB10 interacts directly with OGT in HCC cell lines, Meanwhile, O-GlcNAcylation enhanced RAB10 protein stability. Furthermore, knockdown of OGT suppressed aggressive behaviors of HCC in vitro and in vivo, while elevated RAB10 reversed these. Taken together, these results indicated that OGT-mediated O-GlcNAcylation stabilized RAB10, thus accelerating HCC progression.

中文翻译：

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RAB10 的 O-GlcNAcylation 促进肝细胞癌的进展。

Ras 相关蛋白 Rab-10 (RAB10) 参与肝细胞癌 (HCC) 的肿瘤发生和进展。在这里，我们发现 RAB10、O-GlcNAc 转移酶 (OGT) 和 O-GlcNAcylation 在 HCC 中被上调。此外，RAB10蛋白水平与OGT的表达呈显着正相关。然后研究了 RAB10 的 O-GlcNAcylation 修饰。在这里，我们发现 RAB10 直接与 HCC 细胞系中的 OGT 相互作用，同时，O-GlcNAcylation 增强了 RAB10 蛋白的稳定性。此外，OGT 的敲低抑制了 HCC 在体外和体内的攻击行为，而升高的 RAB10 则逆转了这些行为。总之，这些结果表明 OGT 介导的 O-GlcNAcylation 稳定了 RAB10，从而加速了 HCC 的进展。