This study aimed to evaluate the efficacy of cryopreserved amniotic membrane (AM) grafts in chronic wound healing, including the mean percentage of wound closure per one AM application, and to determine whether the healing efficiency differs between AM grafts obtained from different placentas. A retrospective study analyzing inter-placental differences in healing capacity and mean wound closure after the application of 96 AM grafts prepared from nine placentas. Only the placentas from which the AM grafts were applied to patients suffering from long-lasting non-healing wounds successfully healed by AM treatment were included. The data from the rapidly progressing wound-closure phase (p-phase) were analyzed. The mean efficiency for each placenta, expressed as an average of wound area reduction (%) seven days after the AM application (baseline, 100%), was calculated from at least 10 applications. No statistical difference between the nine placentas’ efficiency was found in the progressive phase of wound healing. The 7-day average wound reduction in particular placentas varied from 5.70 to 20.99% (median from 1.07 to 17.75) of the baseline. The mean percentage of wound surface reduction of all analyzed defects one week after the application of cryopreserved AM graft was 12.17 ± 20.12% (average ± SD). No significant difference in healing capacity was observed between the nine placentas. The data suggest that if there are intra- and inter-placental differences in AM sheets’ healing efficacy, they are overridden by the actual health status of the subject or even the status of its individual wounds.

本研究旨在评估冷冻羊膜 (AM) 移植物在慢性伤口愈合中的功效，包括每次应用 AM 膜时伤口闭合的平均百分比，并确定从不同胎盘获得的 AM 移植物之间的愈合效率是否存在差异。一项回顾性研究分析了应用由 9 个胎盘制备的 96 个 AM 移植物后胎盘间愈合能力和平均伤口闭合的差异。仅包括将 AM 移植物应用于患有长期不愈合伤口且经 AM 治疗成功治愈的患者的胎盘。分析了快速进展的伤口闭合阶段（p 阶段）的数据。每个胎盘的平均效率，表示为 AM 应用后 7 天伤口面积减少的平均值 (%)（基线，100%），是根据至少 10 次应用计算得出的。在伤口愈合的进展阶段，九个胎盘的效率没有发现统计学差异。特定胎盘的 7 天平均伤口减少量为基线的 5.70% 至 20.99%（中位数为 1.07% 至 17.75%）。应用冷冻保存的 AM 移植物一周后，所有分析缺陷的伤口表面减少的平均百分比为 12.17 ± 20.12%（平均值 ± SD）。九个胎盘之间的愈合能力没有观察到显着差异。数据表明，如果 AM 片材的愈合功效存在胎盘内和胎盘间差异，那么这些差异会被受试者的实际健康状况甚至个别伤口的状况所覆盖。