The study aimed to determine the effects of two differing amino acid beverage interventions on biomarkers of intestinal epithelial integrity and systemic inflammation in response to an exertional-heat stress challenge. One week after the initial assessment, participants (n = 20) were randomly allocated to complete two exertional-heat stress trials, with at least 1 week washout. Trials included a water control trial (CON), and one of two possible amino acid beverage intervention trials (VS001 or VS006). On VS001 (4.5 g/L) and VS006 (6.4 g/L), participants were asked to consume two 237-ml prefabricated doses daily for 7 days before the exertional-heat stress, and one 237-ml dose immediately before, and every 20 min during 2-hr running at 60% maximal oxygen uptake in 35 °C ambient conditions. A water volume equivalent was provided on CON. Whole blood samples were collected pre-, immediately post-, 1 and 2 hr postexercise, and analyzed for plasma concentrations of cortisol, intestinal fatty acid protein, soluble CD14, and immunoglobulin M (IgM) by ELISA, and systemic inflammatory cytokines by multiplex. Preexercise resting biomarker concentrations for all variables did not significantly differ between trials (p > .05). A lower response magnitude for intestinal fatty acid protein (mean [95% CI]: 249 [60, 437] pg/ml, 900 [464, 1,336] pg/ml), soluble CD14 (−93 [−458, 272] ng/ml, 12 [−174, 197] ng/ml), and IgM (−6.5 [−23.0, 9.9] MMU/ml, −10.4 [−16.2, 4.7] MMU/ml) were observed on VS001 and V006 compared with CON (p < .05), respectively. Systemic inflammatory response profile was lower on VS001, but not VS006, versus CON (p < .05). Total gastrointestinal symptoms did not significantly differ between trials. Amino acid beverages’ consumption (i.e., 4.5–6.4 g/L), twice daily for 7 days, immediately before, and during exertional-heat stress ameliorated intestinal epithelial integrity and systemic inflammatory perturbations associated with exercising in the heat, but without exacerbating gastrointestinal symptoms.

该研究旨在确定两种不同的氨基酸饮料干预措施对肠上皮完整性和全身炎症生物标志物的影响，以应对劳累性热应激挑战。初次评估一周后，参与者 ( n = 20）被随机分配完成两项劳累热应激试验，至少有 1 周的清除期。试验包括一项水控制试验 (CON) 和两项可能的氨基酸饮料干预试验之一（VS001 或 VS006）。在 VS001 (4.5 g/L) 和 VS006 (6.4 g/L) 中，参与者被要求在劳累性热应激前 7 天每天服用两份 237 毫升预制剂量，并在运动前立即服用一剂 237 毫升，并且每次在 35 °C 环境条件下以 60% 最大摄氧量运行 2 小时期间运行 20 分钟。CON 上提供了水体积当量。运动前、运动后即刻、运动后 1 小时和 2 小时收集全血样本，并通过 ELISA 分析皮质醇、肠脂肪酸蛋白、可溶性 CD14 和免疫球蛋白 M (IgM) 的血浆浓度，并通过多重分析分析全身炎症细胞因子。p  > .05)。肠道脂肪酸蛋白的响应幅度较低（平均 [95% CI]：249 [60, 437] pg/ml、900 [464, 1,336] pg/ml）、可溶性 CD14 (−93 [−458, 272] ng与 VS001 和 V006 相比，在 VS001 和 V006 上观察到 IgM（-6.5 [-23.0, 9.9] MMU/ml、-10.4 [-16.2, 4.7] MMU/ml）和 IgM（-6.5 [-23.0, 9.9] MMU/ml）分别为CON ( p  < .05)。与 CON 相比，VS001 的全身炎症反应较低，但 VS006 则不然（p < .05)。试验之间的总胃肠道症状没有显着差异。在劳累热应激之前和期间摄入氨基酸饮料（即 4.5–6.4 g/L），每天两次，持续 7 天，可改善肠上皮完整性和与高温运动相关的全身炎症扰动，但不会加剧胃肠道反应症状。