Exosome-delivered long non-coding RNAs have a role in the cancer control. It is unknown how exosomal LINC01140 contributes to the breast cancer (BC) growth. The purpose of this investigation is to identify exosomal LINC01140's function in the development of breast cancer. Using quantitative reverse transcripion polymerase chain reaction, the expression of LINC01140 was measured. To investigate how LINC01140 overexpression impacts BC cell proliferation, CCK-8 as well as colony formation assays (CFA) were employed. The expression of apoptosis-related proteins (Bax and Bcl-2) and Wnt/β-catenin signal pathway-related proteins (Wnt, C-myc, β-catenin, and p-GSK-3β) was assessed through Western blotting. Exosomes from BC cells were verified by western blotting to measure CD63 and CD9 levels. To examine how exosomal LINC01140 affects Wnt/β-catenin signaling pathway and xenograft tumor in nude mice, BC cell exosomes that were overexpressing LINC01140 were obtained and co-cultured with BC cells. In BC, it was discovered that LINC01140 had poor expression. BC cell proliferation was inhibited by overexpressing LINC01140, and the levels of the proteins Bcl-2, β-catenin, C-myc, and Wnt were lowered while Bax and p-GSK-3 were increased. In addition, exosomal LINC01140 hindered the activation of the Wnt/β-catenin signaling pathway, leading to a decrease in the growth of breast cancer cells in vivo. The presence of exosomal LINC01140 impedes the initiation of Wnt/β-catenin and reduces the cancerous characteristics of BC cells.

中文翻译：

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外泌体介导的 lncRNA LINC01140 通过调节 Wnt/β-Catenin 通路减缓乳腺癌进展

外泌体递送的长非编码RNA在癌症控制中发挥作用。目前尚不清楚外泌体 LINC01140 如何促进乳腺癌 (BC) 的生长。本研究的目的是确定外泌体 LINC01140 在乳腺癌发展中的功能。使用定量逆转录聚合酶链反应测量LINC01140的表达。为了研究 LINC01140 过度表达如何影响 BC 细胞增殖，采用了 CCK-8 以及集落形成测定 (CFA)。通过Western blotting评估凋亡相关蛋白（Bax和Bcl-2）和Wnt/β-catenin信号通路相关蛋白（Wnt、C-myc、β-catenin和p-GSK-3β）的表达。来自 BC 细胞的外泌体通过蛋白质印迹法进行验证，以测量 CD63 和 CD9 水平。为了研究外泌体LINC01140如何影响裸鼠中Wnt/β-catenin信号通路和异种移植肿瘤，获得过表达LINC01140的BC细胞外泌体并与BC细胞共培养。在BC，发现LINC01140表达较差。过表达LINC01140可抑制BC细胞增殖，Bcl-2、β-catenin、C-myc和Wnt蛋白水平降低，而Bax和p-GSK-3蛋白水平升高。此外，外泌体LINC01140阻碍了Wnt/β-catenin信号通路的激活，导致体内乳腺癌细胞生长减少。外泌体 LINC01140 的存在阻碍了 Wnt/β-catenin 的启动并降低了 BC 细胞的癌性特征。