Beta-sitosterol is a common and useful ‘phytosterol’, present in roots and small branches of Oroxylum indicum (L.) Kurz It has mainly cholesterol-lowering, anti-inflammatory, anti-cancer, and dietary properties. O. indicum is a rare medicinal plant in the traditional medicine system. This study comparatively identified and estimated beta-sitosterol in the methanolic extracts of roots and the small branches of O. indicum by the development of a validated HPTLC method. Chromatographic separation was performed on precoated TLC plates by toluene: ethyl acetate: formic acid: water (6:3:1:0.2, v/v/v/v) with standard procedures using HPTLC assembly. Marker was identified at RF 0.60 as prominent bands and quantified at 540 nm with sharp peaks corresponding to beta-sitosterol by calibration method. The method was validated as per ICH guidelines with a good linear relationship i.e. r² = 0.9973 ± 0.000405 in 100 – 500 ng band⁻¹ with respect to the peak area. The LOD and LOQ were calculated as 35.22 and 106.73 ng/band, respectively. The quantity of marker in extracts of roots and the small branches was found to be 268.7% w/w and 208.5% w/w respectively in 0.5 g of the sample. The method was simple, accurate, and reproducible for rapid screening and quantification of beta-sitosterol in roots and aerial parts of O. indicum. The study may helpful for plant part substitution study, medicinal practises, sustainability, and conservation of such vulnerable plant species. It may useful to establish the quality and efficacy of plant in traditional applications and in routine standardization (quality control) of different commercial preparations of O. indicum.

β-谷甾醇是一种常见且有用的“植物甾醇”，存在于Oroxylum indicum (L.) Kurz的根和小枝中。它主要具有降低胆固醇、抗炎、抗癌和饮食特性。O. indicum是传统药材体系中的珍稀药用植物。本研究通过开发经过验证的 HPTLC方法，比较确定和估计了O. indicum的根和小枝的甲醇提取物中的 β-谷甾醇。甲苯：乙酸乙酯在预涂薄层色谱板上进行色谱分离：甲酸: 水 (6:3:1:0.2, v/v/v/v) 使用 HPTLC 组装的标准程序。标记在 RF 0.60 处被识别为显着条带，并通过校准方法在 540 nm 处量化，具有对应于 β-谷甾醇的尖峰。该方法根据 ICH 指南进行了验证，具有良好的线性关系，即 r ² = 0.9973 ± 0.000405 在 100 – 500 ng 波段^-1中相对于峰面积。计算得出的 LOD 和 LOQ 分别为 35.22 和 106.73 ng/条带。在 0.5 g 样品中，根提取物和小树枝提取物中标记物的含量分别为 268.7% w/w 和 208.5% w/w。该方法简单、准确且重现性好，可快速筛选和定量O. indicum根和地上部分中的 β-谷甾醇. 该研究可能有助于植物部分替代研究、医学实践、可持续性和此类脆弱植物物种的保护。它可能有助于确定植物在传统应用中的质量和功效，以及在O. indicum的不同商业制剂的常规标准化（质量控制）中。