Aims

A hyperinflammatory condition is brought on by the development of Coronavirus disease 2019 (COVID-19), which is characterized by an elevation of T helper (Th) 17 cells, high levels of pro-inflammatory cytokines, and a depletion of regulatory T (Treg) cells.

Methods

In this research, we examined the effect of nano-curcumin and catechin on the TCD4+, TCD8+, Th17, and Treg cells and their associated factors in COVID-19 patients. For this purpose, 160 (50 patients excluded during the study) COVID-19 patients were divided into four groups: placebo, nano-curcumin, catechin, and nano-curcumin + catechin. The frequency of TCD4+, TCD8+, Th17, and Treg cells, the gene expression of transcription factors (STAT3, RORt, and FoxP3) relevant to Th17 and Treg, as well as the serum levels of cytokines (IL-6, IL17, IL1-b, IL-10, and TGF-), were all evaluated intra- and inter-group, before and after treatment, in all groups.

Results

Our study showed that TCD4 + and TCD8 + cells were significantly higher in the nano-curcumin + catechin group compared to the control group, whereas Th17 was lower than the initial value. Furthermore, compared to the placebo-received group, cytokines and transcription factors associated with Th17 were significantly lower in the nano-curcumin + catechin group. Additionally, combined therapy increased Treg cells and transcription factors compared to the placebo group.

Conclusion

Overall, our results show that combining nano-curcumin with catechin has a more notable impact on the enhancement of TCD4+, TCD8+, and Treg cells, as well as a decrease in Th17 cells and their mediators, suggesting a promising combination therapy in reducing the inflammatory conditions of COVID-19 infected patients.

中文翻译：

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纳米姜黄素与儿茶素相结合可改善 COVID-19 感染患者的炎症状况

目标

2019 年冠状病毒病 (COVID-19) 的发展会导致过度炎症，其特点是辅助性 T (Th) 17 细胞升高、促炎细胞因子水平高以及调节性 T (Treg) 细胞耗竭。 ） 细胞。

方法

在这项研究中，我们研究了纳米姜黄素和儿茶素对 COVID-19 患者 TCD4+、TCD8+、Th17 和 Treg 细胞及其相关因素的影响。为此，160 名（研究期间排除了 50 名患者）COVID-19 患者被分为四组：安慰剂组、纳米姜黄素组、儿茶素组和纳米姜黄素+儿茶素组。TCD4+、TCD8+、Th17、Treg细胞的频率，Th17、Treg相关转录因子（STAT3、RORt、FoxP3）基因表达量，以及血清细胞因子（IL-6、IL17、IL1- b、IL-10 和 TGF-) 均在所有组的治疗前和治疗后进行了组内和组间评估。

结果

我们的研究表明，与对照组相比，纳米姜黄素+儿茶素组的TCD4 + 和TCD8 + 细胞显着升高，而Th17 低于初始值。此外，与安慰剂组相比，纳米姜黄素+儿茶素组中与 Th17 相关的细胞因子和转录因子显着降低。此外，与安慰剂组相比，联合治疗增加了 Treg 细胞和转录因子。

结论

总体而言，我们的结果表明，纳米姜黄素与儿茶素的组合对 TCD4+、TCD8+ 和 Treg 细胞的增强以及 Th17 细胞及其介质的减少具有更显着的影响，这表明在减少炎症方面是一种有前景的联合疗法。 COVID-19 感染患者的状况。