Selenium nanoparticles (SeNPs), due to their unique properties, have attracted researchers’ attention. Though SeNPs have been used for wide applications, the chemically synthesized one lacks stability due to aggregation, and it releases toxic byproducts. These drawbacks can be overcome by producing SeNPs using natural sources as reducing and capping agents. Luffa cylindrica is an immense source of phytochemical compounds reported for its potential therapeutical value towards cancer, asthma, and sinusitis. In the current study, we have synthesized SeNPs using leaf extract of L. cylindrica and evaluated its biocompatibility and haemocompatibility using peripheral blood mononuclear cells and erythrocytes respectively. The formation of SeNPs was confirmed by a color change from greenish yellow to ruby red during 6 h incubation at 40${}^{\circ }$C and further confirmed by the maximum absorbance at 266$$nm and 380$$nm in the UV–Vis spectrum. The fingerprint regions of the Fourier-transform infrared spectroscopy spectrum between 1500$$cm${}^{-1}$ and 500$$cm${}^{-1}$ revealed the presence of phytoconstituents of L. cylindrica. The particle size analysis showed a size range of 100$$nm and zeta potential of −13.6 mV. Scanning electron micrograph showed flower-shaped surface morphology with a size range of 100$$nm. The erythrocytes treated with higher concentrations of LC-SeNPs showed less than 5% lysis compared to the positive control. Similarly, in the apoptosis assay, 80.45% of cells remained viable after being treated with LC-SeNPs, which is comparable with that of untreated control. Since the synthesized SeNPs possess biocompatibility and are less cytotoxic, they could be used in cardiac tissue engineering applications. However, further in vitro and in vivo studies are required to confirm its role in cardiac tissue engineering.

硒纳米粒子（SeNPs）由于其独特的性质，引起了研究人员的关注。尽管SeNPs已得到广泛应用，但化学合成的SeNPs由于聚集而缺乏稳定性，并且会释放有毒副产物。这些缺点可以通过使用天然来源作为还原剂和封端剂生产 SeNP 来克服。丝瓜是植物化学化合物的巨大来源，据报道其对癌症、哮喘和鼻窦炎具有潜在的治疗价值。在当前的研究中，我们利用L. cylindrica的叶提取物合成了 SeNPs并分别用外周血单个核细胞和红细胞评价其生物相容性和血液相容性。在 40 ℃ 下孵育 6 小时期间，颜色从绿黄色变为红宝石色，证实了 SeNP 的形成。${}^{\circ }$C并通过266处的最大吸光度进一步证实$$纳米和380$$紫外-可见光谱中的 nm。傅里叶变换红外光谱的指纹区域在 1500 之间$$厘米${}^{-1}$和 500$$厘米${}^{-1}$揭示了L. cylindrica植物成分的存在。粒度分析显示粒度范围为 100$$nm 和 zeta 电位为-13.6 mV。扫描电子显微照片显示花状表面形貌，尺寸范围为100$$纳米。与阳性对照相比，用较高浓度的 LC-SeNP 处理的红细胞显示出小于 5% 的裂解。同样，在细胞凋亡测定中，用 LC-SeNPs 处理后，80.45% 的细胞仍然存活，这与未处理的对照相当。由于合成的 SeNP 具有生物相容性且细胞毒性较小，因此可用于心脏组织工程应用。然而，需要进一步的体外和体内研究来证实其在心脏组织工程中的作用。