This study investigates the potential of cannabidiol (CBD), one major cannabinoid of the plant Cannabis sativa, alone and in combination with a terpene-enriched extract from Humulus lupulus (“Hops 1”), on the LPS-response of RAW 264.7 macrophages as an established in vitro model of inflammation. With the present study, we could support earlier findings of the anti-inflammatory potential of CBD, which showed a dose-dependent [0–5 µM] reduction in nitric oxide and tumor necrosis factor-alpha (TNF-α) released by LPS-stimulated RAW 264.7 macrophages. Moreover, we observed an additive anti-inflammatory effect after combined CBD [5 µM] and hops extract [40 µg/mL] treatment. The combination of CBD and Hops 1 showed effects in LPS-stimulated RAW 264.7 cells superior to the single substance treatments and akin to the control hydrocortisone. Furthermore, cellular CBD uptake increased dose-dependently in the presence of terpenes from Hops 1 extract. The anti-inflammatory effect of CBD and its cellular uptake positively correlated with terpene concentration, as indicated by comparison with a hemp extract containing both CBD and terpenes. These findings may contribute to the postulations for the so-called “entourage effect” between cannabinoids and terpenes and support the potential of CBD combined with phytomolecules from a non-cannabinoid source, such as hops, for the treatment of inflammatory diseases.

这项研究调查了大麻二酚 (CBD) 的潜力，大麻是大麻植物的一种主要大麻素，单独使用以及与来自蛇麻属的富含萜烯的提取物结合使用(“啤酒花 1”)，关于 RAW 264.7 巨噬细胞的 LPS 反应作为已建立的体外炎症模型。通过本研究，我们可以支持 CBD 抗炎潜力的早期发现，该发现显示 LPS 释放的一氧化氮和肿瘤坏死因子-α (TNF-α) 呈剂量依赖性 [0-5 µM] 减少-刺激 RAW 264.7 巨噬细胞。此外，我们观察到 CBD [5 µM] 和啤酒花提取物 [40 µg/mL] 联合处理后的附加抗炎作用。CBD 和啤酒花 1 的组合在 LPS 刺激的 RAW 264.7 细胞中表现出优于单一物质处理的效果，类似于对照氢化可的松。此外，在来自 Hops 1 提取物的萜烯存在下，细胞 CBD 摄取量呈剂量依赖性增加。与含有 CBD 和萜烯的大麻提取物相比，CBD 的抗炎作用及其细胞摄取与萜烯浓度呈正相关。这些发现可能有助于假设大麻素和萜烯之间所谓的“随行效应”，并支持 CBD 与非大麻素来源的植物分子（如啤酒花）结合治疗炎症性疾病的潜力。