Hair loss affects millions of people at some time in their life, and safe and efficient treatments for hair loss are a significant unmet medical need. We report that topical delivery of quercetin (Que) stimulates resting hair follicles to grow with rapid follicular keratinocyte proliferation and replenishes perifollicular microvasculature in mice. We construct dynamic single-cell transcriptome landscape over the course of hair regrowth and find that Que treatment stimulates the differentiation trajectory in the hair follicles and induces an angiogenic signature in dermal endothelial cells by activating HIF-1α in endothelial cells. Skin administration of a HIF-1α agonist partially recapitulates the pro-angiogenesis and hair-growing effects of Que. Together, these findings provide a molecular understanding for the efficacy of Que in hair regrowth, which underscores the translational potential of targeting the hair follicle niche as a strategy for regenerative medicine, and suggest a route of pharmacological intervention that may promote hair regrowth.

中文翻译：

---

单细胞分析揭示了槲皮素在促进毛发再生方面的强大作用。

脱发会影响数百万人一生中的某个时期，而安全有效的脱发治疗是一项未得到满足的重大医疗需求。我们报告说，槲皮素 (Que) 的局部递送刺激静息毛囊随着毛囊角质形成细胞的快速增殖而生长，并补充小鼠毛囊周围的微血管系统。我们构建了毛发再生过程中的动态单细胞转录组景观，发现 Que 治疗刺激毛囊中的分化轨迹，并通过激活内皮细胞中的 HIF-1α 诱导真皮内皮细胞中的血管生成特征。HIF-1α 激动剂的皮肤给药部分概括了 Que 的促血管生成和毛发生长作用。总之，这些发现提供了对 Que 在头发再生中功效的分子理解，