Diclazuril is a classic anticoccidial drug. The key molecules of diclazuril in anticoccidial action allows target screening for the development of anticoccidial drugs. Cyclin-dependent kinases (CDK) are prominent target proteins in apicomplexan parasites. In this study, a diclazuril anticoccidiosis animal model was established, and the transcription and translation levels of the CDK-related kinase 2 of Eimeria tenella (EtCRK2) were detected. mRNA and protein expression levels of EtCRK2 decreased in the infected/diclazuril group compared with those in the infected/control group. In addition, immunofluorescence analysis showed that EtCRK2 was localised in the cytoplasm of the merozoites. The fluorescence intensity of EtCRK2 in the infected/diclazuril group was significantly weaker than that in the infected/control group. The anticoccidial drug diclazuril against E.tenella affects the expression pattern of EtCRK2 molecule, and EtCRK2 is a potential target for new drug development.

地克珠利是一种经典的抗球虫药。地克珠利抗球虫作用的关键分子可以为抗球虫药物的开发进行靶标筛选。细胞周期蛋白依赖性激酶 (CDK) 是顶复门寄生虫中的重要靶蛋白。本研究建立地克珠利抗球虫病动物模型，检测柔嫩艾美耳球虫CDK相关激酶2（ Et CRK2）的转录和翻译水平。与感染/对照组相比，感染/地克珠利组中Et CRK2 mRNA 和蛋白表达水平此外，免疫荧光分析显示Et CRK2定位于裂殖子的细胞质中。感染/地克珠利组中Et CRK2的荧光强度明显弱于感染/对照组。抗球虫药物地克珠利会影响Et CRK2 分子的表达模式， Et CRK2 是新药开发的潜在靶点。