C–H bond oxidation using molecular oxygen as a terminal oxidant is an important reaction in molecular conversions. This reaction is achieved by several enzymes such as cytochrome P450s in biological systems, whereas artificial catalytic systems for this reaction are limited. In this work, the oxidation of ethylbenzene was promoted by a hybrid catalysis system consisting of myoglobin reconstituted with manganese porphycene as an artificial peroxygenase in combination with PdAu nanoparticles encapsulated in hollow mesoporous silica spheres as a solid catalyst to produce hydrogen peroxide, respectively. Neither catalyst alone provides hydroxylated products. Favorable conditions for hydrogen peroxide generation and subsequent C–H bond hydroxylation are different from each other and require optimization of pH and salt concentration. The optimized conditions are found to be 0.5 atm of H₂ and 0.5 atm of O₂ at pH 8.5 in the presence of 10 mM NaCl. The total optimized turnover number of the hybrid catalysis system for ethyl benzene hydroxylation is 3.6, which is consistent with 97% of the turnover number value of the same reaction catalyzed by reconstituted myoglobin using 0.5 mM hydrogen peroxide under the hydrogen-oxygen mixed atmosphere. This finding indicates that the hybrid catalyst system operates without any negative effects for both catalytic reactions.

使用分子氧作为末端氧化剂的C-H键氧化是分子转化中的重要反应。该反应是通过生物系统中的细胞色素P450等多种酶来实现的，而用于该反应的人工催化系统是有限的。在这项工作中，通过混合催化系统促进乙苯的氧化，该混合催化系统由用锰卟啉重构的肌红蛋白作为人工过氧化酶，与封装在中空介孔二氧化硅球中的PdAu纳米颗粒作为固体催化剂相结合，分别产生过氧化氢。两种催化剂均不能单独提供羟基化产物。过氧化氢生成和随后的 C-H 键羟基化的有利条件各不相同，需要优化 pH 值和盐浓度。₂和 0.5 atm O ₂，pH 8.5，存在 10 mM NaCl。该混合催化体系乙苯羟基化的总优化周转数为3.6，与在氢氧混合气氛下使用0.5 mM过氧化氢重构肌红蛋白催化相同反应的周转数97%一致。这一发现表明混合催化剂系统的运行对两种催化反应没有任何负面影响。