Genetic modification of rice is mainly carried out by Agrobacterium-mediated transformation of callus accompanied by tissue culture. It is time consuming, laborious and unapplicable for cultivars unable to induce callus. In this study, we have reported a novel gene transfer protocol that involves pulling out primary leaf from coleoptile and injection of Agrobacterium culture into the empty channel. Out of 25 plants survived after injection of Agrobacterium tumefaciens EHA105 culture harboring pCAMBIA1301-RD29A-AtDREB1A, 8 T₀ plants revealed the expected size of around 811 bp corresponding to AtDREB1A gene and Southern blotting analysis on 18 T₁ plants suggested introgression of AtDREB1A. 3 T₂ lines (7–9, 12–3, 18–6) exhibited accumulation of free proline and soluble sugars, yet increase of chlorophyll content, but decrease of electrolyte leakage and methane dicarboxylic aldehyde under cold stress condition at the vegetative growth stage. Yield components investigation on T₂ lines showed earlier heading date and no yield loss compared to wild type plants grown under normal condition. GUS expression analysis and integrated transgene detection in T₀ and T₁ plants followed by evaluation of cold stress tolerance in T₂ lines suggest the advantage of this in planta transformation protocol to obtain transgenic rice.

水稻的遗传修饰主要是通过农杆菌介导的愈伤组织转化并伴有组织培养来进行。费时费力，对于不能诱导愈伤组织的品种不适用。在这项研究中，我们报道了一种新颖的基因转移方案，该方案涉及从胚芽鞘中拔出初生叶并将农杆菌培养物注射到空通道中。在注射含有pCAMBIA1301-RD29A- AtDREB1A的根癌农杆菌EHA105 培养物后，25 株植物中存活，8 株 T _{0植物显示出与}AtDREB1A基因相对应的约 811 bp 的预期大小，对 18 株 T ₁植物的 Southern 印迹分析表明AtDREB1A基因渗入。3 T ₂品系（7-9、12-3、18-6）在营养生长阶段的冷胁迫条件下，游离脯氨酸和可溶性糖积累，叶绿素含量增加，但电解质渗漏和甲烷二醛减少。_{对 T 2}品系的产量组成调查显示，与正常条件下生长的野生型植物相比，抽穗日期更早，并且没有产量损失。_{T 0}和T ₁植物中的GUS表达分析和整合转基因检测，随后评估T _{2系中的冷胁迫耐受性表明这}在植物转化方案中获得转基因水稻的优势。