Recent work in biosensors has shown promise to enable high throughput searches through large genetic libraries. However, just as physiological limitations and lack of in-depth mechanistic knowledge can prevent us from achieving high titers in microbial systems, similar roadblocks can appear in the application of biosensors. Here, we characterized a previously developed transcription-factor (ExuR) based galacturonate biosensor for its other cognate ligand, glucuronate. Though we saw an ideal response to glucuronate from the biosensor in controlled and ideal experimental circumstances, these results began to deviate from a well-behaved system when we explored the application of the sensor to different MIOX homologs. Through modifications to circuit architecture and culture conditions, we were able to decrease this variation and use these more optimal conditions to apply the biosensor for the separation of two closely related MIOX homologs.

中文翻译：

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用于单细胞检测葡萄糖醛酸的生物传感器的开发

最近在生物传感器方面的工作已显示出通过大型基因库实现高通量搜索的希望。然而，正如生理限制和缺乏深入的机械知识会阻止我们在微生物系统中实现高滴度一样，生物传感器的应用也可能出现类似的障碍。在这里，我们表征了先前开发的基于转录因子（ExuR）的半乳糖醛酸生物传感器，用于其其他同源配体葡萄糖醛酸。尽管我们在受控和理想的实验环境中看到生物传感器对葡萄糖醛酸的理想响应，但当我们探索传感器对不同 MIOX 同系物的应用时，这些结果开始偏离表现良好的系统。通过修改电路架构和培养条件，