Introduction Bioactive proteins and peptides generated from fruit, vegetables, meat or fish have great potential as functional food or substitutes for antibiotics. In recent years it has also been demonstrated that the fungus kingdom could be a source of these compounds. The study investigated the bioactivity of an extract of the lignicolous fungus Trametes versicolor and its hydrolysate. Material and Methods The fungus was collected in a mixed forest in October, extracted and hydrolysed. To inspect the protein and peptide profiles before and after hydrolysis, matrix-assisted laser desorption/ionisation–time-of-flight mass spectrometric analysis was performed. To evaluate the antioxidant properties of the preparations, 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+) radical scavenging assays were used. The activity of the fungus extract and hydrolysate against Aeromonas veronii, Bacillus cereus, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella Typhimurium, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, Streptococcus dysgalactiae, and Streptococcus uberis was determined by the minimum inhibitory concentration and minimum bactericidal concentration values. Results The extract and its hydrolysate showed almost 100% ABTS•+ and DPPH• radical scavenging with a low half maximal inhibitory concentration. The water extract and hydrolysate of T. versicolor exhibited antimicrobial activity against two S. aureus strains, E. coli, P. aeruginosa and Salmonella Typhimurium. Conclusion These results provide compelling evidence that the analysed fungus extract and its hydrolysate hold promise with their antibacterial and antioxidant properties.

中文翻译：

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从云芝中提取的富含蛋白质的提取物的抗氧化和抗菌特性

简介 从水果、蔬菜、肉类或鱼类中产生的生物活性蛋白质和肽具有作为功能性食品或抗生素替代品的巨大潜力。近年来，还证明真菌王国可能是这些化合物的来源。该研究调查了木质真菌提取物的生物活性花斑栓菌及其水解产物。材料与方法 10月份在混交林中采集真菌，进行提取和水解。为了检查水解前后的蛋白质和肽谱，进行了基质辅助激光解吸/电离-飞行时间质谱分析。为了评价制剂的抗氧化性能，2,2-二苯基-1-三硝基苯肼（DPPH•) 和 2,2′-连氮基双(3-乙基苯并噻唑啉-6-磺酸) (ABTS•+）使用自由基清除测定。真菌提取物和水解物的活性维氏气单胞菌,蜡样芽孢杆菌,粪肠球菌,屎肠球菌,大肠杆菌,肺炎克雷伯菌,铜绿假单胞菌,沙门氏菌鼠伤寒杆菌，金黄色葡萄球菌,表皮葡萄球菌,无乳链球菌,停乳链球菌， 和乳房链球菌由最低抑菌浓度和最低杀菌浓度值确定。结果 提取物及其水解物显示几乎 100% ABTS•+和DPPH•具有低半数抑制浓度的自由基清除作用。水提取物和水解物云芝对两种物质表现出抗菌活性金黄色葡萄球菌菌株，大肠杆菌,铜绿假单胞菌和沙门氏菌鼠伤寒杆菌。结论 这些结果提供了令人信服的证据，证明所分析的真菌提取物及其水解产物具有抗菌和抗氧化特性。