Abstract

Since the nanoscale in combination with luminescent properties and prospective applications in different fields of optoelectronics and biomedicine is stimulating a growing interest towards research in the area of cadmium telluride (CdTe) quantum dots (QDs), the method of “green” synthesis of CdTe QDs with the use of the Pleurotus ostreatus mycelium culture as a biological matrix has been developed. The study of their physical and chemical characteristics has shown that the synthesized CdTe QDs are characterized by a crystalline structure and a predominantly spherical morphology and are 3–8 nm in size with the luminescence maximum within the 340–370 nm range. The study of their effects on different types of mammalian cells has shown that CdTe QDs have dose-dependent effects on mouse endothelial cells and human and rat erythrocytes, T- and B-lymphocytes, colorectal cancer cells (Colo 205), and human breast cancer cells (MCF-7). In particular, suppression of proliferative indices of endotheliocytes and an increase in the count of dead cells were observed, which indicates the cytotoxic action of nanocrystalline CdTe and its antiproliferative effect on endothelial cells. At the 5 µM concentration, CdTe QDs exhibited hemolytic activity, due to their action on erythrocytes, and affected adhesive contacts and cancer cell survivability. At the same time, human breast cancer cells (MCF-7) were more sensitive to their action. The data obtained are exclusively important for the understanding of mechanisms underlying the toxicity of CdTe QDs and for their future application in biological and biomedical research.

中文翻译：

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CdTe 量子点的绿色合成及其对人类和动物细胞的影响

摘要

由于纳米级结合发光特性和在光电子学和生物医学不同领域的潜在应用正在激发人们对碲化镉 (CdTe) 量子点 (QD) 领域的研究越来越感兴趣，因此“绿色”合成 CdTe QD 的方法使用平菇已经开发出菌丝体培养物作为生物基质。对其物理和化学特性的研究表明，合成的 CdTe QD 具有晶体结构和主要球形形态的特征，尺寸为 3-8 nm，发光最大值在 340-370 nm 范围内。对它们对不同类型哺乳动物细胞的影响的研究表明，CdTe QD 对小鼠内皮细胞和人类和大鼠红细胞、T 和 B 淋巴细胞、结直肠癌细胞 (Colo 205) 和人类乳腺癌具有剂量依赖性影响细胞 (MCF-7)。特别是观察到内皮细胞增殖指数的抑制和死细胞计数的增加，这表明纳米晶 CdTe 的细胞毒作用及其对内皮细胞的抗增殖作用。在 5 µM 浓度下，CdTe QD 表现出溶血活性，这是由于它们对红细胞的作用，并影响粘附接触和癌细胞的生存能力。同时，人类乳腺癌细胞 (MCF-7) 对其作用更加敏感。获得的数据对于理解 CdTe QD 毒性的潜在机制及其在生物学和生物医学研究中的未来应用非常重要。