Zinc finger CCHC-type containing 4 (ZCCHC4), RNA binding protein, has been reported to mediate rRNA methylation and affect tumor cell proliferation. However, the role of ZCCHC4 in the regulation of osteosarcoma (OS) remains unknown. ZCCHC4 was highly expressed in OS tissues and cell lines. Overexpression or silencing of ZCCHC4 promoted or inhibited cell proliferation, epithelial-mesenchymal transition (EMT), and motility. Additionally, we proved that ZCCHC4 facilitates OS progression through upregulating integrin β1 (ITGB1). In the animal model, ZCCHC4 knockdown reduced OS tumor growth and metastases in vivo. Our findings showed that ZCCHC4 promoted the progression of OS through upregulating ITGB1 and suggested that inhibition of ZCCHC4 could be a novel therapeutic strategy for OS.

中文翻译：

---

ZCCHC4 通过上调 ITGB1 促进骨肉瘤进展

据报道，含有 4 (ZCCHC4) 的锌指 CCHC 型 RNA 结合蛋白可介导 rRNA 甲基化并影响肿瘤细胞增殖。然而，ZCCHC4 在骨肉瘤 (OS) 调节中的作用仍不清楚。ZCCHC4 在 OS 组织和细胞系中高表达。ZCCHC4 的过度表达或沉默会促进或抑制细胞增殖、上皮间质转化 (EMT) 和运动。此外，我们证明 ZCCHC4 通过上调整合素 β1 (ITGB1) 促进 OS 进展。在动物模型中，ZCCHC4 敲低可减少体内OS 肿瘤的生长和转移。我们的研究结果表明，ZCCHC4 通过上调 ITGB1 促进 OS 的进展，并表明抑制 ZCCHC4 可能是 OS 的一种新治疗策略。